Artificial Corn-Based Diet for Rearing Spodoptera frugiperda (Lepidoptera: Noctuidae)
Artificial Corn-Based Diet for Rearing Spodoptera frugiperda (Lepidoptera: Noctuidae)
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DOI：
10.1093/jisesa/iez052
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发表时间：
2019-07-01
影响因子：
2.2
通讯作者：
De Bortoli, Sergio Antonio
中科院分区：
文献类型：
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作者：
Lima Pinto, Jose Ricardo;Torres, Aline Finotti;De Bortoli, Sergio Antonio
草地贪夜蛾Spodoptera frugiperda（J.E. Smith，1797）被认为是玉米的主要害虫。然而，在实验室中用于饲养这种昆虫的人工饲料不含玉米。本研究的目的是评估生物学和比较的食物消耗的S。frugiperda，以及幼虫在标准饮食和玉米为基础的饮食的食物偏好。评价了以下三种饮食：基于豆类的标准饮食（D-1）、用玉米粉替代小麦胚芽的饮食（D-2）和用绿色玉米替代豆类的饮食（D-3）。评价的生物学参数是幼虫和蛹的时期和存活率;雄性和雌性蛹的重量;性比;繁殖力;卵孵化期;和成虫寿命。营养指标的测定和获得的生物学数据被用来确定生育力生命表的参数，我们还进行了多项选择试验（喂养试验）。S. frugiperda发生在所有三个饮食，虽然没有产卵的女性从幼虫喂养D-2。在生育力生命表参数方面，饮食之间没有差异。饮食D-2导致更好的摄取、消化、同化和食物转化，但与同化食物的代谢成本相关。使用多项选择测试，我们观察到，幼虫更喜欢饮食D-2。根据上述结果，提出了饲养黄颡鱼的最适饲料。实验室条件下对草地贪小蜂的最佳防治效果为D-1和D-3
Spodoptera frugiperda (J.E. Smith, 1797) is considered a key pest of maize. However, the artificial diets used for rearing this insect in the laboratory do not contain corn. The aim of this study was to evaluate the biology and to compare the food consumption by S. frugiperda, as well as the food preference of the larvae in the standard diet and the corn-based diet. Three of the following diets were evaluated: a standard diet based on beans (D-1), a diet with corn flour as substitute for wheat germ (D-2), and a diet replacing beans with green corn (D-3). The biological parameters evaluated were period and survival of larvae and pupae; weight of male and female pupae; sex ratio; fecundity; egg incubation period; and adult longevity. The nutritional indices were determined and the biological data obtained were used to determine the parameters of fertility life tables; we also performed a multiple-choice test (feeding test). Larval development of S. frugiperda occurred in all three diets, although without oviposition by females developed from larval fed on D-2. There was no difference among the diets in relation to the fertility life table parameters. The diet D-2 resulted in better ingestion, digestion, assimilation, and conversion of food, but was associated with a metabolic cost to assimilate the food. Using a multiple-choice test, we observed that the larvae preferred diet D-2. Based on our results, the most adequate diets for rearing S. frugiperda in the laboratory are D-1 and D-3