The Association of Leucocyte Telomere Length with Non-alcoholic Fatty Liver Disease in Type 2 Diabetes Patients
The Association of Leucocyte Telomere Length with Non-alcoholic Fatty Liver Disease in Type 2 Diabetes Patients
复制标题
2 型糖尿病患者白细胞端粒长度与非酒精性脂肪肝的关系
DOI：
10.1097/cm9.0000000000000559
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发表时间：
2019
影响因子：
6.1
通讯作者：
kun dong
中科院分区：
文献类型：
--
作者：
Min Zhang;Manli Hu;Jiaojiao Huang;Sanshan Xia;Yan Yang;kun dong
背景：白细胞端粒已被证明与胰岛素抵抗相关疾病有关，如2型糖尿病（T2DM）和非酒精性脂肪性肝病（NAFLD）。本横断面研究探讨了T2DM患者白细胞端粒长度与NAFLD的关系。方法：收集120例无NAFLD的T2DM患者和120例年龄匹配的T2DM合并NAFLD患者的临床特征，采用以southern southern印迹法为金标准的末端限制性内切片段长度分析法测定白细胞端粒长度（LTL）。结果：伴有NAFLD的T2DM患者LTL明显长于无NAFLD的T2DM患者（6400.2±71.8 bp vs 6023.7±49.5 bp, P <0.001），特别是当糖尿病病程小于2年时。同时，T2DM合并NAFLD患者LTL变短的趋势与糖尿病持续时间增加相关，而非NAFLD患者LTL变短的趋势与糖尿病持续时间增加相关。最后，LTL、体重指数、甘油三酯与T2DM患者的NAFLD状态有显著相关性。结论：T2DM合并NAFLD患者的LTL明显长于非NAFLD患者。较长的LTL在T2DM早期尤为明显，表明较长的LTL可作为T2DM患者NAFLD的生物标志物。
Background: Leucocyte telomere has been shown to be related to insulin resistance-related disease, such as type 2 diabetes mellitus (T2DM) and non-alcoholic fatty liver disease (NAFLD). This cross-sectional study investigated the association of leucocyte telomere length with NAFLD in T2DM patients..Methods: Clinical features were collected and Leukocyte leukocyte telomere length (LTL) was measured by southern Southern blot-based terminal restriction fragment length analysis method considered as the gold standard in 120 T2DM patients without NAFLD and 120 age-matched T2DM patients with NAFLD. NAFLD was clinically defined as by manifestations of B ultrasonography..Results: LTL in T2DM patients with NAFLD were significantly longer than those without NAFLD (6400.2 ± 71.8 bp versus 6023.7 ± 49.5 bp, P <0.001), especially when diabetes duration was less than 2 years. Meanwhile, the trend of shorter LTL was associated with the increased diabetes duration in T2DM patient with NAFLD, but not in T2DM patients without NAFLD. Finally, LTL, as well as body mass index, triglycerides, had significant association with NAFLD status in T2DM patients..Conclusions: T2DM patients with NAFLD had a significantly longer LTL than those who without NAFLD. The longer LTL was especially evident in the early stage of T2DM, indicating that longer LTL may be used as a biomarker for NAFLD in T2DM patients.