Promoter activity of the retinoic acid receptor gene in the Ciona intestinalis embryo
Promoter activity of the retinoic acid receptor gene in the Ciona intestinalis embryo
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DOI：
10.1002/dvdy.20265
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发表时间：
2005-04-01
影响因子：
2.5
通讯作者：
Fujiwara, S
中科院分区：
文献类型：
--
作者：
Fujiwara, S
视黄酸调节许多脊索动物特异性特征的发育。编码视黄酸受体（RAR）的基因仅从脊索动物中分离出来。在本研究中，基因组DNA片段含有RAR基因的启动子区（Ci-RAR）在原脊索动物玻璃海鞘分离。含有1，326 bp的Ci-RAR 5 '侧翼区的转基因在表皮、中枢神经系统和肌肉中表达。在表皮中表达所必需的序列元件似乎被细分为两个元件。一个是所需的激活背中线表皮和其他的表皮。负责中枢神经系统激活的元件的特征在于Fox、Ets和加塔转录因子的推定结合位点。尾肌细胞中的转基因表达需要E-box序列。(c)2005 Wiley-Liss，Inc.
Retinoic acid regulates development of many chordate-specific characteristics. The genes encoding retinoic acid receptors (RARs) were isolated only from chordates. In the present study, a genomic DNA fragment containing the promoter region of the RAR gene (Ci-RAR) in the protochordate Ciona intestinalis was isolated. The transgene containing 1,326 bp of the Ci-RAR 5'flanking region was expressed in the epidermis, central nervous system, and muscle. The sequence element necessary for expression in the epidermis seemed to be subdivided into two elements. One was required for activation in the dorsal midline epidermis and the other for the rest of the epidermis. The element responsible for activation in the central nervous system was characterized by putative binding sites for Fox, Ets, and GATA transcription factors. An E-box sequence was required for the transgene expression in the tail muscle cells.(c) 2005 Wiley-Liss, Inc.