Accelerating Biphasic Biocatalysis through New Process Windows.
Accelerating Biphasic Biocatalysis through New Process Windows.
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DOI：
10.1002/anie.202005183
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发表时间：
2020-09-14
期刊：
影响因子：
--
通讯作者：
Wirth T
中科院分区：
文献类型：
--
作者：
Huynh F;Tailby M;Finniear A;Stephens K;Allemann RK;Wirth T
通过连续流动反应的过程强化提高了精细化学品和药品的生产速度。催化反应是通过一种非常规的和前所未有的使用高效液相/液相逆流色谱系统加速。产物的生成明显快于传统间歇式反应器或分段流系统，这是通过立体选择相转移催化反应的例子。该方法还可以加强生物催化作用，如在高产量酯化和倍半萜环化酶催化下从法尼酯二磷酸合成倍半萜作为高价值的天然产物，在医药、农业和香料工业中应用。倍半萜合成酶中的产物释放由于倍半萜的疏水性而受到速率限制，但暴露在离心力下的双相系统允许高效反应。高效液相/液相逆流色谱系统的新应用证明了双相反应的高反应速率和优异收率，例如立体选择相转移催化反应和倍半萜的生物催化合成。HPCCC系统的催化速度比间歇式反应器快70倍，比分段流系统快10倍，且产率接近定量。
Process intensification through continuous flow reactions has increased the production rates of fine chemicals and pharmaceuticals. Catalytic reactions are accelerated through an unconventional and unprecedented use of a high‐performance liquid/liquid counter current chromatography system. Product generation is significantly faster than in traditional batch reactors or in segmented flow systems, which is exemplified through stereoselective phase‐transfer catalyzed reactions. This methodology also enables the intensification of biocatalysis as demonstrated in high yield esterifications and in the sesquiterpene cyclase‐catalyzed synthesis of sesquiterpenes from farnesyl diphosphate as high‐value natural products with applications in medicine, agriculture and the fragrance industry. Product release in sesquiterpene synthases is rate limiting due to the hydrophobic nature of sesquiterpenes, but a biphasic system exposed to centrifugal forces allows for highly efficient reactions. A new application of high‐performance liquid/liquid counter current chromatography system demonstrated high reaction rates and excellent yields for biphasic reactions, exemplified with steroselective phase‐transfer catalysed reactions and biocatalytic synthesis of sesquiterpenes. Catalysis in the HPCCC system was up to 70 times faster than in batch reactors and up to 10 times faster than in segmented flow systems with nearly quantitative yields.
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影响因子：
6.1
作者：
Burns, JR;Ramshaw, C
通讯作者：
Ramshaw, C
影响因子：
3.4
作者：
Cascon, Oscar;Richter, Gerald;Wirth, Thomas
通讯作者：
Wirth, Thomas
DOI：
10.1080/10826079208016340
发表时间：
1992-01-01
期刊：
JOURNAL OF LIQUID CHROMATOGRAPHY
影响因子：
--
作者：
ITO, Y
通讯作者：
ITO, Y
DOI：
10.1002/anie.201609557
发表时间：
2017-04-03
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Demiray M;Tang X;Wirth T;Faraldos JA;Allemann RK
通讯作者：
Allemann RK
影响因子：
15
作者：
Jin, YH;Williams, DC;Coates, RM
通讯作者：
Coates, RM