An assessment of the clonality of the components of canine mixed mammary tumours by mitochondrial DNA analysis
An assessment of the clonality of the components of canine mixed mammary tumours by mitochondrial DNA analysis
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DOI：
10.1016/j.tvjl.2008.07.005
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发表时间：
2009-11-01
影响因子：
2.2
通讯作者：
Cassali, Geovanni D.
中科院分区：
文献类型：
--
作者：
Bertagnolli, Angelica C.;Soares, Paula;Cassali, Geovanni D.
本研究的目的是调查，如果在犬乳腺混合瘤的线粒体DNA（mtDNA）的D-环片段控制区的突变可以被用作克隆标记，确定细胞群体的起源。对10个良性混合性乳腺肿瘤和9个由良性混合性肿瘤产生的癌进行显微解剖，并检查来自上皮和间充质肿瘤细胞以及来自正常乳腺组织的DNA的高变控制区片段中的序列变异。（以及在相应的正常组织中）在80%的良性混合瘤和89%的由良性混合瘤产生的癌中发现，表明共享克隆起源。在15.8%的上皮和间充质成分中发现的独特序列改变，在所有19个肿瘤检查中，表明少数乳腺肿瘤起源于多克隆或发生早期克隆分歧的可能性。未观察到混合肿瘤成分的mtDNA D环片段内突变增加。(C)2008爱思唯尔有限公司保留所有权利。
The aim of this study was to investigate if mutations in the mitochondrial DNA (mtDNA) D-loop fragment control region of canine mammary mixed tumours could be used as clonal markers that identified the cell population of origin. Ten benign mixed mammary tumours and nine carcinomas arising from benign mixed tumours were microdissected and DNA from epithelial and mesenchymal tumour cells and from normal mammary tissue was examined for sequence variations in a fragment of the hypervariable control region.Identical sequence variants in both the epithelial and triesenchymal components (as well as in the corresponding normal tissue) were found in 80% of the benign mixed tumours and in 89% of the carcinomas arising from benign mixed tumours suggesting a shared clonal origin. The distinctive sequence alterations identified in the epithelial and mesenchymal components of 15.8%, of all 19 tumours examined, suggests the possibility that a minority of mammary tumours are polyclonal in origin or that early clonal divergence occurs. Increased mutation within the mtDNA D-loop fragment of mixed tumour components was not observed. (C) 2008 Elsevier Ltd. All rights reserved.