pH-regulated chloride secretion in fetal lung epithelia.
pH-regulated chloride secretion in fetal lung epithelia.
复制标题
胎儿肺上皮细胞中 pH 调节的氯离子分泌。
DOI：
10.1152/ajplung.2000.278.6.l1248
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Zeitlin,PL
中科院分区：
文献类型：
--
作者：
Blaisdell,CJ;Edmonds,RD;Wang,XT;Guggino,S;Zeitlin,PL
胎肺主动转运氯离子通过气道上皮。ClC-2是一种pH激活的氯离子通道，在胎儿肺中高度表达，位于发育中的呼吸上皮的顶面。我们的目标是确定酸性pH值是否可以刺激安装在Ussing室中的胎鼠远端肺上皮细胞的氯化物分泌。一系列酸性溶液刺激等效短路电流（Ieq），从基线28 ± 4.8（pH 7.4）至70 ± 5（pH 6.2）、114 ± 12.8（pH 5.0）和164 ± 19.2（pH 3.8）μA/cm 2。1 mM氯化镉可抑制Ieq中的这些变化，且不会导致[3 H]甘露醇细胞旁通量的大的变化。共聚焦显微镜免疫荧光检测显示，ClC-2表达沿着极化胎儿远端肺上皮细胞的腔表面。这些数据表明，胎儿肺液的酸性环境可以激活氯离子通道，促进胎儿肺液的产生，这些Ussing研究中观察到的变化可能是由于ClC-2的刺激。
The fetal lung actively transports chloride across the airway epithelium. ClC-2, a pH-activated chloride channel, is highly expressed in the fetal lung and is located on the apical surface of the developing respiratory epithelium. Our goal was to determine whether acidic pH could stimulate chloride secretion in fetal rat distal lung epithelial cells mounted in Ussing chambers. A series of acidic solutions stimulated equivalent short-circuit current (Ieq) from a baseline of 28 ± 4.8 (pH 7.4) to 70 ± 5 (pH 6.2), 114 ± 12.8 (pH 5.0), and 164 ± 19.2 (pH 3.8) μA/cm2. These changes inIeqwere inhibited by 1 mM cadmium chloride and did not result in large changes in [3H]mannitol paracellular flux. Immunofluorescent detection by confocal microscopy revealed that ClC-2 is expressed along the luminal surface of polarized fetal distal lung epithelial cells. These data suggest that the acidic environment of the fetal lung fluid could activate chloride channels contributing to fetal lung fluid production and that the changes inIeqseen in these Ussing studies may be due to stimulation of ClC-2.