Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape.
Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape.
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DOI：
10.1016/j.jhazmat.2010.12.069
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发表时间：
2011-02
影响因子：
13.6
通讯作者：
YAN-FENG Zhang;Linyan He;Zhao-jin Chen;Wen-hui Zhang;Qing-ya Wang;Meng Qian;X. Sheng
中科院分区：
文献类型：
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作者：
YAN-FENG Zhang;Linyan He;Zhao-jin Chen;Wen-hui Zhang;Qing-ya Wang;Meng Qian;X. Sheng
从铅锌尾矿上生长的鸭跖草（Commelina communis）植株中分离到49株耐铅内生细菌，初步筛选出7株产ACC脱氨酶的内生细菌，并对其进行了抗重金属、产ACC脱氨酶、吲哚乙酸（IAA）和铁载体等特性的研究。在盆栽条件下，研究了两个ACC脱氨酶活性较高的菌株（Q2 BJ 2和Q2 BG 1）在0和100 mgkg − 1铅的石英砂中对油菜生长和铅吸收的促进作用。7个耐Pb和产ACC脱氨酶的内生细菌菌株被发现表现出不同的多重重金属抗性特征，并显示出不同水平的ACC脱氨酶活性（范围从12.8μM α-KB mg−1h− 1到121μM α-KB mg−1h−1）。在7个菌株中，6个菌株产生吲哚乙酸，而5个菌株产生铁载体。在实验中，油菜植株生长在含有100 mgkg − 1铅的石英砂中，与未接种的对照相比，接种分离物导致地上组织（范围从39%到71%）和根（范围从35%到123%）的干重增加。在100 mgkg − 1铅污染基质中培养的油菜地上部组织铅含量增加58%至62%。
Forty-nine lead (Pb)-resistant endophytic bacteria were isolated from metal-tolerant Commelina communis plants grown on lead and zinc mine tailing, of which, seven 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophytic bacteria were initially obtained and characterized with respect to heavy metal resistance and production of ACC deaminase, indole-3-acetic acid (IAA) as well as siderophores. Two isolates (Q2BJ2 and Q2BG1) showing higher ACC deaminase activity were evaluated for promoting plant growth and Pb uptake of rape grown in quartz sand containing 0 and 100mgkg−1of Pb in pot experiments. The seven Pb-resistant and ACC deaminase-producing endophytic bacterial isolates were found to exhibit different multiple heavy metal resistance characteristics and to show different levels of ACC deaminase activity (ranging from 12.8μM α-KB mg−1h−1to 121μM α-KB mg−1h−1). Among the seven isolates, six isolates produced indole acetic acid, whilst five isolates produced siderophores. In experiments involving rape plants grown in quartz sand containing 100mgkg−1of Pb, inoculation with the isolates resulted in the increased dry weights of above-ground tissues (ranging from 39% to 71%) and roots (ranging from 35% to 123%) compared to the uninoculated control. Increases in above-ground tissue Pb contents of rape cultivated in 100mgkg−1of Pb-contaminated substrates varied from 58% to 62% in inoculated-rape plants compared to the uninoculated control.