Methods for measuring gluconeogenesis in vivo.
Methods for measuring gluconeogenesis in vivo.
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DOI：
10.1097/00075197-199809000-00016
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发表时间：
1998-09-01
影响因子：
3.1
通讯作者：
Brunengraber, H
中科院分区：
文献类型：
--
作者：
Previs, S F;Brunengraber, H
稳定同位素技术的最新发展已经导致了用于测量人类中葡萄糖生成的贡献的新方案的概念。早期的技术受到变量低估导致同位素交换过程中发生的碳标记从示踪剂到葡萄糖。本文综述了四种新技术：（1）[~（13）C]甘油或[~（13）C]乳酸盐标记葡萄糖的质量同位素分布分析，（2）[U-~（13）C_6]葡萄糖输注过程中葡萄糖和乳酸盐的质量同位素分布分析，（3）[U-~（13）C_6]葡萄糖的质量同位素分布分析，（4）[U-~（13）C_6]葡萄糖的质量同位素分布分析。（3）通过摄入~ 2 H_2 O使体内水富集~ 2 H，并测定葡萄糖C_5和C_2上的~ 2 H标记，（4）通过核磁共振波谱测量的葡萄糖周转率和肝糖原分解率之间的差异。四个协议的优点和局限性进行了讨论。2 H2O技术是最实用的;它不受同位素交换产生的伪影的影响，也不受肝脏代谢分区的影响。
Recent developments in stable isotope technology have led to the conception of new protocols for measuring the contribution of gluconeogenesis to glucose production in humans. Earlier techniques were subject to variable underestimations resulting from isotopic exchanges occurring during the transfer of carbon label from the tracer to glucose. This review concentrates on four novel techniques: (1) mass isotopomer distribution analysis of glucose labelled from [13C]glycerol or [13C]lactate; (2) mass isotopomer distribution analysis of glucose and lactate during infusion of [U-13C6]glucose; (3) 2H-enrichment of body water by ingestion of 2H2O, and measuring the 2H-labelling on C5 and C2 of glucose, and (4) difference between glucose turnover and rate of hepatic glycogenolysis measured by nuclear magnetic resonance spectroscopy. The advantages and limitations of the four protocols are discussed. The 2H2O technique is the most practical; it is not subject to artifacts resulting from isotopic exchanges, and is not affected by zonation of hepatic metabolism.