Tissue-specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species.
Tissue-specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species.
复制标题
几种昆虫物种线粒体磷酸盐载体旁系同源物的组织特异性表达和沉默表型。
DOI：
10.1111/imb.12297
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发表时间：
2017
影响因子：
2.6
通讯作者：
H Miyoshi and T Shiotsuki
中科院分区：
文献类型：
--
作者：
R Sugahara;A Jouraku;T Nakakura;M Minaba;Takenori Yamamoto;Yasuo Shinohara;H Miyoshi and T Shiotsuki
线粒体磷酸盐载体基因（PiC）编码一种膜蛋白，介导无机磷酸盐从细胞质供应到线粒体基质中。这种底物特异性转运系统在有效的ATP合成中起着重要作用。哺乳动物似乎只有一个具有两个选择性剪接变体的PiC，其功能差异尚不清楚。目前的研究是第一个描述昆虫中编码PiC的多个基因的研究。在家蚕中发现了两个PiC旁系同源物，一个是普遍存在的，一个是睾丸特异性的，后者似乎只存在于鳞翅目中。在果蝇中发现了两个PiC旁系同源物，而另一种双翅目昆虫中国斑潜蝇有三个PiC旁系同源物。在Plautia stali中发现了两个PiCs，并且沉默这两个基因中的任何一个都会影响Plautia stali的正常发育。Stalinopathy的PiC基因在不同组织中的表达模式不同，Schistocerca gregaria和Locusta migratoria各有两个PiC基因，表达模式不同，赤拟谷盗只有一个PiC基因，在幼虫发育过程中起重要作用。因此，尽管无机磷酸盐转运系统在真核生物中似乎是保守的，但PiC在不同昆虫物种的不同组织中变得特化。
The mitochondrial phosphate carrier gene (PiC) encodes a membrane protein that mediates the supply of inorganic phosphate from the cytosol into the mitochondrial matrix. This substrate‐specific transport system plays an important role in efficient ATP synthesis. Mammals appear to have only onePiCwith two alternative splicing variants whose functional differences remain unclear. The present study is the first to characterize the multiple genes that encode PiC in insects.Bombyx moriwas found to have twoPiCparalogues, one ubiquitous and one testis‐specific, the latter seeming to be present only in Lepidoptera.Drosophila melanogasterwas found to harbour twoPiCparalogues, whereasLiriomyza chinensis, another dipteran, has threePiCparalogues. TwoPiCswere found to be present inPlautia stali, and silencing either of these genes affected the normal development ofP. stalinymphs, although their expression patterns differed amongst tissues.Schistocerca gregariaandLocusta migratoriahave twoPiCeach, with different expression patterns.Tribolium castaneumwas found to have only onePiC, which appears to play an essential role in larval development. Thus, although the inorganic phosphate transport system appears to be conserved across eukaryotes,PiChas become specialized in the different tissues of different insect species.