The HC-Pro cistron of Triticum mosaic virus is dispensable for systemic infection in wheat but is required for symptom phenotype and efficient genome amplification.
The HC-Pro cistron of Triticum mosaic virus is dispensable for systemic infection in wheat but is required for symptom phenotype and efficient genome amplification.
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DOI：
10.1016/j.virusres.2023.199277
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发表时间：
2024-01-02
期刊：
影响因子：
5
通讯作者：
Kovacs, Frank
中科院分区：
文献类型：
--
作者：
Tatineni, Satyanarayana;Alexander, Jeffrey;Kovacs, Frank
关键词：
HC-Pro-顺反子对于TriMV在小麦中的系统感染是消耗性的。HC-Pro-是TriMV症状表型和基因组RNA拷贝和外壳蛋白的有效积累所必需的。包含HC-Pro的aa 5-25中的缺失引起与TriMV-ΔHC-Pro相似的表型。在TriMV中缺失3-50或3-125氨基酸的HC-Pro-引起严重的症状表型，基因组RNA积累增加，但CP降低。来自马铃薯Y病毒属或小麦病毒属的杂合HC-Pros不能补充症状表型和病毒滴度。小麦花叶病毒（Triticum mosaic virus，TriMV）是马铃薯Y病毒科（Potyviridae）Poacevirus属的模式种，是美国大平原地区小麦曲螨传播的一种重要经济病毒。本研究利用反向遗传学方法对TriMV编码的辅助组分蛋白酶（HC-Pro）的功能基因组学进行了研究。完全缺失HC-Pro顺反子的TriMV在小麦中引起系统感染，表明HC-Pro顺反子对于TriMV系统感染是不利的。然而，缺乏HC-Pro的TriMV引起具有轻微症状的延迟系统感染，这导致植物很少或没有发育迟缓，基因组RNA拷贝和外壳蛋白（CP）的积累显著减少。从TriMV基因组中HC-Pro顺反子5′端开始的连续缺失突变显示，除了氨基酸3和4之外，氨基酸3至25内的缺失引起轻微症状，基因组RNA和CP积累减少。令人惊讶的是，在HC-Pro中缺失氨基酸3至50或3至125的TriMV引起严重的症状，基因组RNA拷贝大幅增加，但CP积累急剧减少。此外，含有来自其他马铃薯Y属的异源HC-Pro的TriMV产生的症状表型和基因组RNA积累与不含HC-Pro的TriMV相似，这表明其他马铃薯Y属的HC-Pro不能有效补充小麦中的TriMV。我们的数据表明HC-Pro对于TriMV的复制是消耗性的，但对于有效的病毒基因组RNA扩增和症状发展是必需的。在HC-Pro顺反子中具有各种缺失的TriMV的可用性将有助于检查小麦曲螨传播对HC-Pro的需求。
The HC-Pro-cistron is expendable for TriMV systemic infection in wheat. HC-Pro-is required for TriMV symptom phenotype and efficient accumulation of genomic RNA copies and coat protein. Deletions in HC-Pro-comprising aa 5–25 elicited phenotypes similar to TriMV-ΔHC-Pro. HC-Pro-with deletion of aa 3–50 or 3–125 in TriMV elicited severe symptom phenotype with increased accumulation of genomic RNA but decreased CP. Heterologous HC-Pros from potyvirus or tritimovirus genera did not complement for symptom phenotype and virus titer. Triticum mosaic virus (TriMV), the type species of the genus Poacevirus in the family Potyviridae, is an economically important wheat curl mite-transmitted wheat-infecting virus in the Great Plains region of the USA. In this study, the functional genomics of helper component-proteinase (HC-Pro) encoded by TriMV was examined using a reverse genetics approach. TriMV with complete deletion of HC-Pro cistron elicited systemic infection in wheat, indicating that HC-Pro cistron is dispensable for TriMV systemic infection. However, TriMV lacking HC-Pro caused delayed systemic infection with mild symptoms that resulted in little or no stunting of plants with a significant reduction in the accumulation of genomic RNA copies and coat protein (CP). Sequential deletion mutagenesis from the 5′ end of HC-Pro cistron in the TriMV genome revealed that deletions within amino acids 3 to 25, except for amino acids 3 and 4, elicited mild symptoms with reduced accumulation of genomic RNA and CP. Surprisingly, TriMV with deletion of amino acids 3 to 50 or 3 to 125 in HC-Pro elicited severe symptoms with a substantial increase in genomic RNA copies but a drastic reduction in CP accumulation. Additionally, TriMV with heterologous HC-Pro from other potyvirids produced symptom phenotype and genomic RNA accumulation similar to that of TriMV without HC-Pro, suggesting that HC-Pros of other potyvirids were not effective in complementing TriMV in wheat. Our data indicate that HC-Pro is expendable for replication of TriMV but is required for efficient viral genomic RNA amplification and symptom development. The availability of TriMV with various deletions in the HC-Pro cistron will facilitate the examination of the requirement of HC-Pro for wheat curl mite transmission.
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影响因子：
14.9
作者：
Firth AE
通讯作者：
Firth AE
影响因子：
3.7
作者：
Kasschau, KD;Cronin, S;Carrington, JC
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DOI：
10.1073/pnas.95.22.13079
发表时间：
1998-10-27
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