THE V-REL ONCOGENE ENCODES A KAPPA-B ENHANCER BINDING-PROTEIN THAT INHIBITS NF-KAPPA-B FUNCTION
THE V-REL ONCOGENE ENCODES A KAPPA-B ENHANCER BINDING-PROTEIN THAT INHIBITS NF-KAPPA-B FUNCTION
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DOI：
10.1016/0092-8674(90)90146-6
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发表时间：
1990-11-16
期刊：
影响因子：
64.5
通讯作者：
GREENE, WC
中科院分区：
文献类型：
--
作者：
BALLARD, DW;WALKER, WH;GREENE, WC
NF-κ B的研究表明，该增强子结合活性对应于在T细胞活化期间用双相动力学差异诱导的至少四种蛋白质（p50、p55、p75和p85）的家族。虽然p55和p50与NF-κ B的50 kd DNA结合亚基密切相关，但p75和p85显示出DNA结合特性，使它们与该50 kd多肽及其调节亚基I κ B和p65区别开来。该κ B特异性蛋白家族的所有四个成员在结构上与v-Rel癌蛋白相关，并且其中一个p85似乎与人c-Rel相同。v-Rel而不是非转化性v-Rel突变体结合κ B增强子并抑制NF-κ B激活的从IL-2受体α的转录。启动子和HIV-1 LTR。这些发现提示了Rel-相关家族的κ B增强子结合蛋白，并提高了v-Rel的转化活性与其对NF-κ B控制下的细胞基因的抑制作用相关的可能性。
Studies of NF-.kappa.B suggest that this enhancer binding activity corresponds to a family of at least four proteins (p50, p55, p75, and p85) differentially induced with biphasic kinetics during T cell activation. While p55 and p50 are closely related to the 50 kd DNA binding subunit of NF-.kappa.B, p75 and p85 exhibit DNA binding properties that distinguish them from this 50 kd polypeptide and its regulatory subunits I.kappa.B and p65. All four members of this .kappa.B-specific protein family are structurally related to the v-Rel oncoprotein and one, p85, appears identical to human c-Rel. v-Rel, but not nontransforming v-Rel mutants, binds to the .kappa.B enhancer and inhibits the NF-.kappa.B-activated transcription from the IL-2 receptor .alpha. promoter and HIV-1 LTR. These findings suggest a Rel-related family of .kappa.B enhancer binding proteins and raise the possibility that the transforming activity of v-Rel is linked to its inhibitory action on cellular genes under NF-.kappa.B control.