Homozygous diploid deletion strains of Saccharomyces cerevisiae that determine lag phase and dehydration tolerance.
Homozygous diploid deletion strains of Saccharomyces cerevisiae that determine lag phase and dehydration tolerance.
复制标题
决定滞后期和脱水耐受性的酿酒酵母纯合二倍体缺失菌株。
DOI：
10.1007/s00253-004-1793-1
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Hammond,TimothyG
中科院分区：
文献类型：
--
作者：
D'Elia,Riccardo;Allen,PatriciaL;Johanson,Kelly;Nickerson,CherylA;Hammond,TimothyG
This study identifies genes that determine length of lag phase, using the model eukaryotic organism,Saccharomyces cerevisiae. We report growth of a yeast deletion series following variations in the lag phase induced by variable storage times after drying-down yeast on filters. Using a homozygous diploid deletion pool, lag times ranging from 0 h to 90 h were associated with increased drop-out of mitochondrial genes and increased survival of nuclear genes. Simple linear regression (R2analysis) shows that there are over 500 genes for which >70% of the variation can be explained by lag alone. In the genes with a positive correlation, such that the gene abundance increases with lag and hence the deletion strain is suitable for survival during prolonged storage, there is a strong predominance of nucleonic genes. In the genes with a negative correlation, such that the gene abundance decreases with lag and hence the strain may be critical for getting yeast out of the lag phase, there is a strong predominance of glycoproteins and transmembrane proteins. This study identifies yeast deletion strains with survival advantage on prolonged storage and amplifies our understanding of the genes critical for getting out of the lag phase.
