Inhibin and beta type transforming growth factor (TGF beta) have opposite modulating effects on the follicle stimulating hormone (FSH)-induced aromatase activity of cultured rat granulosa cells.
Inhibin and beta type transforming growth factor (TGF beta) have opposite modulating effects on the follicle stimulating hormone (FSH)-induced aromatase activity of cultured rat granulosa cells.
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DOI：
10.1016/0006-291x(86)90427-4
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发表时间：
1986-05
影响因子：
3.1
通讯作者：
S. Ying;A. Becker;N. Ling;N. Ueno;R. Guillemin
中科院分区：
文献类型：
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作者：
S. Ying;A. Becker;N. Ling;N. Ueno;R. Guillemin
我们最近观察到，阿摩尔浓度的外源添加TGFβ（一种与抑制素结构相关的分子）可以刺激垂体细胞培养物中FSH的基础分泌。从猪卵泡液中纯化的抑制素可拮抗 TGFβ 的这种活性。为了进一步了解抑制素和 TGFβ 的稳态调节特性，我们研究了卵巢颗粒细胞的芳香酶活性是否也受到这些肽的卵巢内调节。将来自未成熟垂体切除的己烯雌酚处理的大鼠的颗粒细胞用雄烯二酮 (10−7M) 作为底物、oFSH (2 ng) 和不同量的 TGFβ 或抑制素培养 2 天。基础雌激素分泌可以忽略不计，并且不受纯化的 TGFβ 或抑制素 (10 ng/ml) 处理的影响，而 oFSH (2 ng/ml) 处理使雌激素积累增加 100 倍。同时应用增加浓度（10 pg - 10 ng/ml）的TGFβ在FSH刺激的雌激素积累中产生剂量依赖性增量，ED5 0 为0.3 ± 0.02 ng/ml。另一方面，FSH 与 10 pg 至 10 ng/ml 范围内的抑制素同时孵育会降低 FSH 介导的雌激素分泌。 TGFβ拮抗抑制素对芳香酶活性的抑制作用。这些发现表明，抑制素和 TGFβ 这两种密切相关的分子在调节卵泡功能中发挥着新颖且相反的作用。
We have recently observed that attomolar concentration of exogenously added TGFβ, a molecule structurally related to inhibin, can stimulate the basal secretion of FSH in a pituitary cell culture. Inhibin purified from porcine follicular fluid antagonizes this activity of TGFβ. To understand further the homeostatic regulatory properties of inhibin and TGFβwe have investigated whether the aromatase activity of ovarian granulosa cells is also subject to intra-ovarian modulation by these peptides.Granulosa cells from immature hypophysectomized diethylstilbestrol-treated rats were cultured for 2 days with androstenedione (10−7M) as a substrate, oFSH (2 ng), and different amounts of TGFβor inhibin. Basal estrogen secretion was negligible and remained unaffected by treatment with purified TGFβor inhibin (10 ng/ml), whereas treatment with oFSH (2 ng/ml) produced a 100-fold increase in estrogen accumulation. The concurrent application of increasing concentrations (10 pg - 10 ng/ml) of TGFβproduced dose-dependent increments in the FSH-stimulated accumulation of estrogen with a ED5 0of 0.3 ± 0.02 ng/ml. On the other hand, concurrent incubation of FSH with inhibin ranging from 10 pg to 10 ng/ml decreases the FSH-mediated estrogen secretion. TGFβantagonizes the inhibition of inhibin on aromatase activity. These findings suggest that inhibin and TGFβ, two closely related molecules, play novel and opposite roles in modulating the follicular functions.