STED super-resolved microscopy
STED super-resolved microscopy
复制标题
DOI：
10.1038/nmeth.4593
复制
发表时间：
2018-03-01
期刊：
影响因子：
48
通讯作者：
Diaspro, Alberto
中科院分区：
文献类型：
--
作者：
Vicidomini, Giuseppe;Bianchini, Paolo;Diaspro, Alberto
受激发射损耗（STED）显微镜提供亚衍射分辨率，同时保留荧光显微镜的有用方面，如光学切片，分子特异性和灵敏度。然而，复杂的显微镜架构和高照明强度限制了STED显微镜在过去的广泛使用。在这里，我们总结的进展，减轻这些问题，并给予实质性的势头STED显微镜应用。我们讨论了这种方法在时空限制，活细胞成像和光谱学相结合方面的未来。这些领域的进展可能会将STED显微镜提升为生命科学中成像的标准方法。
Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical sectioning, and molecular specificity and sensitivity. However, sophisticated microscopy architectures and high illumination intensities have limited STED microscopy's widespread use in the past. Here we summarize the progress that is mitigating these problems and giving substantial momentum to STED microscopy applications. We discuss the future of this method in regard to spatiotemporal limits, live-cell imaging and combination with spectroscopy. Advances in these areas may elevate STED microscopy to a standard method for imaging in the life sciences.