Measuring the effect of glutamate receptor agonists on extracellular D-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis
Measuring the effect of glutamate receptor agonists on extracellular D-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis
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DOI：
10.1016/j.neulet.2005.09.080
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发表时间：
2006-01-30
影响因子：
2.5
通讯作者：
Bowser, MT
中科院分区：
文献类型：
--
作者：
Ciriacks, CM;Bowser, MT
在线微透析-毛细管电泳（CE）被用来测量细胞外D-丝氨酸浓度的变化，响应于应用谷氨酸激动剂和拮抗剂在大鼠纹状体。微透析-CE分析能够测量小至8%的浓度变化，采样速率为12-15 s。红藻氨酸（KA）诱导D-和L-丝氨酸浓度的增加。非NMDA受体拮抗剂CNQX的应用并没有影响观察到的D-或L-丝氨酸的增加，这表明一个非特异性的影响。NMDA还诱导D-丝氨酸、L-丝氨酸、谷氨酸和GABA浓度的增加。这些增加被NMDA受体拮抗剂MK-801减弱。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular D-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in D- and L-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for D- or L-serine, suggesting a nonspecific effect. NMDA also induced increases in D-serine, L-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801. (c) 2005 Elsevier Ireland Ltd. All rights reserved.