Estimating disease resistance in insects:: phenoloxidase and lysozyme-like activity and disease resistance in the cricket Gryllus texensis
Estimating disease resistance in insects:: phenoloxidase and lysozyme-like activity and disease resistance in the cricket Gryllus texensis
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DOI：
10.1016/j.jinsphys.2003.11.011
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发表时间：
2004-02-01
影响因子：
2.2
通讯作者：
Adamo, SA
中科院分区：
文献类型：
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作者：
Adamo, SA
动物抵抗疾病的能力通常通过测量免疫系统的一个或多个组成部分来估计。有一种假设，即这些免疫测定法测量动物产生有效免疫应答的能力。本文通过研究两种常见的估计昆虫免疫活性，酚氧化酶和溶菌酶样酶活性，和抵抗三种常见的昆虫细菌病原体：粘质沙雷氏菌，Serratia acidifaciens，蜡状芽孢杆菌之间的关系来测试这一假设。个体内总酚氧化酶和基线溶菌酶样活性之间存在相关性（斯皮尔曼r（s）= 0.33，p< 0.001，n= 190对）。然而，总酚氧化酶和基线溶菌酶样活性水平并不能预测哪些雄性蟋蟀能在三种细菌的挑战中存活下来。免疫攻击后，溶菌酶样活性增加（Friedman，33.72，p< 0.001），增加越多，蟋蟀在S. marcescens（斜率= 0.15，chi（2）= 8.2，p= 0.005）或B。蜡样变（斜率= 0.8，卡方（2）= 6.4，p= 0.01）。具有较高总血淋巴蛋白浓度的蟋蟀也比具有较低总血淋巴蛋白浓度的蟋蟀更有可能在三种细菌病原体中的任何一种的挑战中存活下来（S。大肠杆菌：斜率= 0.62，卡方（2）= 9.2，p= 0.002; B.蜡样芽胞杆菌：slope = 0.02，chi（2）= 6.5，p= 0.01; marcescens：slope = 0.03，chi（2）= 7.8，p= 0.005）。由于免疫系统的复杂性，经验性试验对免疫力和抵抗力之间关系的测定范围很广。对正确解释这些措施很重要。(C)2003 Elsevier Ltd.保留所有权利。
An animal's ability to resist disease is usually estimated by measuring one or more components of the immune system. There is:an assumption that these assays of immunity measure an animal's ability to mount an effective immune response. This paper tests this assumption by examining the relationship between two common estimates of insect immunocompetence, phenoloxidase and lysozyme-like enzyme activity, and resistance to three common insect bacterial pathogens: Serratia marcescens, Serratia liquefaciens, and Bacillus cereus. There was a correlation (Spearman's r(s) = 0.33, p< 0.001, n= 190 pairs) between total phenoloxidase and baseline lysozyme-like activity within individuals. However, total phenoloxidase and baseline lysozyme-like activity levels did not predict which male crickets would survive any of the three bacterial challenges. Lysozyme-like activity increased after,an immune challenge (Friedman, 33.72, p< 0.001), and the greater the increase, the greater the chance that the cricket would survive S. marcescens (slope = 0.15, chi(2) = 8.2, p= 0.005) or B. cereus (slope = 0.8, chi(2)= 6.4, p= 0.01). The crickets with a greater total hemolymph protein concentration were also more likely to survive a challenge with any of the three bacterial pathogens than the crickets with lower total hemolymph protein concentrations (S. liquefaciens: slope = 0.62, chi(2)= 9.2, p= 0.002; B. cereus: slope = 0.02, chi(2) = 6.5, p= 0.01; S. marcescens: slope = 0.03, chi(2)= 7.8, p= 0.005). Because of the complexity of the immune system, empirical tests of the relationship between assays of immunity and resistance to a range. of actual pathogens are important for correctly interpreting these measures. (C) 2003 Elsevier Ltd. All rights reserved.