Biomechanical behavior of scar tissue and uninjured skin in a porcine model
Biomechanical behavior of scar tissue and uninjured skin in a porcine model
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DOI：
10.1111/j.1524-475x.2009.00463.x
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发表时间：
2009-03-01
影响因子：
2.9
通讯作者：
Hart, David A.
中科院分区：
文献类型：
--
作者：
Corr, David T.;Gallant-Behm, Corrie L.;Hart, David A.
一种测试皮肤轴向和横向拉伸性能的新方法被开发出来，以提高我们对皮肤力学行为的理解，以及它在损伤和疤痕形成后的变化。在母幼猪（N=14）全层损伤后70天对皮肤组织进行评估。在轴向（颅尾）和横向（背腹）方向采集疤痕组织和未损伤皮肤的样本，并在体外使用应力松弛和拉伸破坏的方案进行机械评估。未损伤皮肤在轴向上比在横向上更柔顺，趾入区更大，载荷松弛更快。这种方向差异不存在于高负载响应中，例如线性刚度或失效特性。与未受伤的皮肤相比，疤痕显示出相似的线性刚度，大大降低了破坏特性，降低了低负荷顺应性。伤痕在低负荷行为、粘性响应或失效特性方面没有方向性差异。这些发现表明损伤后可能发生的形态学变化与实验观察到的粘弹性和方向性变化一致。这提高了对损伤如何影响皮肤生物力学功能的理解，为设计成功的移植手术和组织工程皮肤替代物提供了必要的有价值的信息。
A new method to test axial and transverse tensile properties of skin was developed to improve our understanding of skin mechanical behavior, and how it changes following injury and formation of a scar. Skin tissue was evaluated at 70 days following full-thickness wounding in juvenile female pigs (N=14). Samples were taken in the axial (cranial-caudal) and transverse (dorsal-ventral) directions, for both scar tissue and uninjured skin, and were evaluated mechanically in vitro using a protocol of stress relaxation followed by tensile failure. Uninjured skin was more compliant, with a larger toe-in region, and faster load relaxation, in the axial direction than the transverse. Such directional differences were not present in high-load responses, such as linear stiffness or failure properties. When compared with uninjured skin, scars displayed a similar linear stiffness, with considerably reduced failure properties, and reduced low-load compliance. Scars showed no directional differences in low-load behavior, viscous response, or failure properties. These findings suggest morphological changes that may occur with injury that are consistent with the viscoelastic and directional changes observed experimentally. This improved understanding of how injury affects skin biomechanical function provides valuable information necessary for the design of successful grafting procedures and tissue-engineered skin replacements.