Tissue flow regulates planar cell polarity independently of the Frizzled core pathway
Tissue flow regulates planar cell polarity independently of the Frizzled core pathway
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DOI：
10.1016/j.celrep.2022.111388
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发表时间：
2022-09-20
期刊：
影响因子：
8.8
通讯作者：
Yamazaki，Masakazu
中科院分区：
文献类型：
--
作者：
Ayukawa，Tomonori;Akiyama，Masakazu;Yamazaki，Masakazu
平面细胞极性(PCP)调节外部结构的取向。包括Frizzled蛋白质在内的一组核心蛋白质构成了PCP调控系统的核心。其他独立于核心小组的PCP机制也可能存在，但其基本机制难以捉摸。在这里，我们证明了组织流是一种控制果蝇肛门上核心群非依赖性PCP的机制。核心群功能的丧失仅对成人中央鼻孔的刷毛取向有轻微影响。这种近乎正常的PCP是由组织流介导的在蛹阶段随机的刷毛取向的挽救所致。操纵研究表明，组织流动可以使刷毛朝向与流动相反的方向。这一过程独立于核心组，并暗示根尖细胞外基质的功能就像一把“梳子”，以对齐刷毛。我们的结果揭示了组织动力学和细胞外物质在PCP建立过程中的协同作用。
Planar cell polarity (PCP) regulates the orientation of external structures. A core group of proteins that includes Frizzled forms the heart of the PCP regulatory system. Other PCP mechanisms that are independent of the core group likely exist, but their underlying mechanisms are elusive. Here, we show that tissue flow is a mechanism governing core group-independent PCP on theDrosophilanotum. Loss of core group function only slightly affects bristle orientation in the adult central notum. This near-normal PCP results from tissue flow-mediated rescue of random bristle orientation during the pupal stage. Manipulation studies suggest that tissue flow can orient bristles in the opposite direction to the flow. This process is independent of the core group and implies that the apical extracellular matrix functions like a "comb" to align bristles. Our results reveal the significance of cooperation between tissue dynamics and extracellular substances in PCP establishment.