A heterodimeric DNA polymerase: Evidence that members of Euryarchaeota possess a distinct DNA polymerase
A heterodimeric DNA polymerase: Evidence that members of Euryarchaeota possess a distinct DNA polymerase
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DOI：
10.1073/pnas.95.24.14250
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发表时间：
1998-11-24
影响因子：
11.1
通讯作者：
Ishino, Y
中科院分区：
文献类型：
--
作者：
Cann, IKO;Komori, K;Ishino, Y
在这里，我们描述了一个DNA聚合酶家族高度保守的Euryarchaeota，一个亚域的Euryarchaea。DNA聚合酶由两种蛋白质DP1和DP2组成。序列分析表明，DP1和真核生物DNA聚合酶δ的第二亚基之间存在相当大的相似性，真核生物的繁殖所必需的蛋白质，DP2具有核苷酸聚合活性的蛋白质中发现的保守基序。这些结果，加上我们以前的生化分析的成员之一，DNA聚合酶II（DP 1 + DP 2）从Pyrococcus furiosus，牵连这个家庭的DNA聚合酶的DNA复制过程中的广古菌。这个DNA聚合酶家族的发现，除了提供了一个机会，以提高我们的DNA聚合酶的进化知识，是一个重要的一步，在整个生命的三个领域的DNA复制的完整理解。
We describe here a DNA polymerase family highly conserved in Euryarchaeota, a subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2. Sequence analysis showed that considerable similarity exists between DP1 and the second subunit of eukaryotic DNA polymerase delta, a protein essential for the propagation of Eukarya, and that DP2 has conserved motifs found in proteins with nucleotide-polymerizing activity. These results, together with our previous biochemical analyses of one of the members, DNA polymerase II (DP1 + DP2) from Pyrococcus furiosus, implicate the DNA polymerases of this family in the DNA replication process of Euryarchaeota. The discovery of this DNA-polymerase family, aside from providing an opportunity to enhance our knowledge of the evolution of DNA polymerases, is a significant step toward the complete understanding of DNA replication across the three domains of life.