Microscopic detection of thermogenesis in a single HeLa cell
Microscopic detection of thermogenesis in a single HeLa cell
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DOI：
10.1529/biophysj.106.098673
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发表时间：
2007-03-15
影响因子：
3.4
通讯作者：
Ishiwata, Shin'ichi
中科院分区：
文献类型：
--
作者：
Suzuki, Madoka;Tseeb, Vadim;Ishiwata, Shin'ichi
我们在此报告使用一种新近设计的微温度计（一种填充有热敏荧光染料三（2-噻吩甲酰基）三氟丙酮合铕（III）三水合物的玻璃微吸管）检测和测量单个细胞温度变化的技术。我们发现，在离子霉素诱导细胞外空间的Ca2+内流之后，单个海拉细胞的产热会有一定的时间延迟。该时间延迟与细胞外的[Ca2+]呈反比，并且当Ca2+-ATP酶被毒胡萝卜素阻断时，温度升高受到抑制。这些观察结果有力地表明，内质网中Ca2+-ATP酶的酶活性导致了产热。因此，这项研究为在单细胞水平上研究产热作用铺平了道路。
We report here the technique for detection and measurement of the temperature changes in single cells using a recently devised microthermometer (a glass micropipette filled with the thermosensitive fluorescent dye Europium (III) thenoyltrifluoroacetonate trihydrate). We found that the heat production in a single HeLa cell occurred with some time delay after the ionomycin-induced Ca2+ influx from the extracellular space. The time delay inversely depended on extracellular [Ca2+], and the increase in temperature was suppressed when Ca2+-ATPases were blocked by thapsigargin. These observations strongly suggest that the enzymatic activity of Ca2+-ATPases in endoplasmic reticulum leads to the heat production. This study has therefore paved the way for studying the thermogenesis at the single-cell level.