Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo
Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo
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DOI：
10.1538/expanim.59.105
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Okabe, Masaru
中科院分区：
文献类型：
--
作者：
Hasuwa, Hidetoshi;Muro, Yuko;Okabe, Masaru
本文介绍了一种精子顶体表达绿色荧光蛋白（GFP）、线粒体表达红色荧光蛋白（RFP）的转基因小鼠[B6 D2 F1- Tg（CAG/su 9-DsRed 2，Acr 3-EGFP）RBGS 002 Osb]。双荧光精子在体内和体外受精中均表现出正常的受精能力，在解剖出雌性生殖道并置于激发光下时，可透过子宫壁和输卵管壁观察到精子。这一特性有助于检查女性生殖道内的精子迁移，并有助于顶体反应的原位实时成像，其细节仍然难以捉摸。
In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.