Ultrasensitive surface-enhanced Raman scattering detection in common fluids
Ultrasensitive surface-enhanced Raman scattering detection in common fluids
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DOI：
10.1073/pnas.1518980113
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发表时间：
2016-01-12
影响因子：
11.1
通讯作者：
Wong, Tak-Sing
中科院分区：
文献类型：
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作者：
Yang, Shikuan;Dai, Xianming;Wong, Tak-Sing
在任何流体中检测高特异性和高灵敏度的目标分析物对分析科学和技术具有重要意义。表面增强拉曼散射(SERS)已被证明能够检测具有高特异性的单分子，但在任何高度稀释的溶液中实现单分子灵敏度仍然是一个挑战。在这里，我们展示了一个通用平台，它允许分析物在水和非水流体中浓缩并输送到SERS敏感部位，并随后对罗丹明6G(R6G)进行定量检测，其水平降至类似75fM(10(-15)mol.L-1)。我们的平台，称为滑液注入多孔表面增强拉曼散射(SLIPSERS)，基于一种滑的、无所不在的基质，使分析物和SERS基质(例如，金纳米颗粒)能够在蒸发的液滴中完全集中。结合我们的SLIPSERS平台和SERS作图技术，我们系统地量化了在从750fM(p>；90%)到75am(10(-18)mol.L-1)水平(P)的浓度c范围内检测到R6G分子的概率p(C)。
Detecting target analytes with high specificity and sensitivity in any fluid is of fundamental importance to analytical science and technology. Surface-enhanced Raman scattering (SERS) has proven to be capable of detecting single molecules with high specificity, but achieving single-molecule sensitivity in any highly diluted solutions remains a challenge. Here we demonstrate a universal platform that allows for the enrichment and delivery of analytes into the SERS-sensitive sites in both aqueous and nonaqueous fluids, and its subsequent quantitative detection of Rhodamine 6G (R6G) down to similar to 75 fM level (10(-15) mol.L-1). Our platform, termed slippery liquid-infused porous surface-enhanced Raman scattering (SLIPSERS), is based on a slippery, omniphobic substrate that enables the complete concentration of analytes and SERS substrates (e.g., Au nanoparticles) within an evaporating liquid droplet. Combining our SLIPSERS platform with a SERS mapping technique, we have systematically quantified the probability, p(c), of detecting R6G molecules at concentrations c ranging from 750 fM (p > 90%) down to 75 aM (10(-18) mol.L-1) levels (p