Solid sampling technique for direct detection of condensed tannins in bark by matrix-assisted laser desorption/ionization mass spectrometry.
Solid sampling technique for direct detection of condensed tannins in bark by matrix-assisted laser desorption/ionization mass spectrometry.
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DOI：
10.1002/rcm.1845
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发表时间：
2005-03
期刊：
影响因子：
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通讯作者：
Y. Ishida;K. Kitagawa;K. Goto;H. Ohtani
中科院分区：
文献类型：
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作者：
Y. Ishida;K. Kitagawa;K. Goto;H. Ohtani
基于基质辅助激光解吸/电离质谱法（MALDI-MS）和简单的固体取样技术，建立了树皮中缩合单宁成分的直接分析方法。从木（树皮）粉样品中获得的MALDI质谱清晰地显示出一系列峰，对应于浓缩单宁低聚物的钠离子加合物，高达m/z 3000左右。结果表明，本研究中所用树皮样品中的缩合单宁主要由黄烷-3-醇单元组成，如proisetinidin （PF）、proprobinetinidin （PR）和prodelphinidin (PD)，至少高达10-mers （m/z约3000）。
A novel method for the direct analysis of condensed tannin components in bark was developed on the basis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a simple solid sampling technique. The MALDI mass spectra obtained from the wood (bark) powder sample clearly showed a series of peaks corresponding to the sodium ion adducts of condensed tannin oligomers up to around m/z 3000. The results indicate that the condensed tannins in the bark sample used in this work mostly consist of combinations of flavan-3-ol units such as profisetinidin (PF), prorobinetinidin (PR) and prodelphinidin (PD), at least up to 10-mers (m/z approximately 3000).