Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways
Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways
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DOI：
10.1016/j.btre.2018.e00296
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发表时间：
2019-03-01
影响因子：
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通讯作者：
Ismail, Maznah
中科院分区：
文献类型：
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作者：
Foo, Su Chern;Yusoff, Fatimah Md.;Ismail, Maznah
本实验研究了C. calcitrans提取物及其富岩藻黄素（fucocanthin rich fraction, FxRF）对人肝癌HepG2细胞的抗增殖作用。通过细胞毒性试验、形态学观察和细胞周期分析确定各提取物的功效。观察到的作用机制通过多重基因表达分析进行评估。结果表明，CME和FxRF对HepG2细胞具有剂量依赖性和时间依赖性。FxRF （IC50: 18.89 mg.mL(-1)）显著高于CME (IC50: 87.5 mg.mL(-1)) （p < 0.05）。基因表达研究表明，C. calcitranans提取物对细胞的抗增殖作用部分是通过调节与细胞信号传导（AKT1, ERK1/2， JNK），细胞凋亡（BAX， BID, Bcl-2， APAF， CYCS）和氧化应激（SOD1, SOD2， CAT）有关的许多基因来介导的。总体而言，C. calcitrans提取物对HepG2癌细胞具有有效的干预作用，随着岩藻黄素含量的增加，凋亡活性增强。(c) 2018年Elsevier B.V.出版
In this study, anti-proliferative effects of C. calcitrans extract and its fucoxanthin rich fraction (FxRF) were assessed on human liver HepG2 cancer cell line. Efficacy from each extract was determined by cytotoxicity assay, morphological observation, and cell cycle analysis. Mechanisms of action observed were evaluated using multiplex gene expression analysis. Results showed that CME and FxRF induced cytotoxicity to HepG2 cells in a dose and time-dependent manner. FxRF (IC50: 18.89 mg.mL(-1)) was found to be significantly more potent than CME (IC50: 87.5 mg.mL(-1)) (p < 0.05). Gene expression studies revealed that anti-proliferative effects in treated cells by C. calcitrans extracts were mediated partly through the modulation of numerous genes involved in cell signaling (AKT1, ERK1/2, JNK), apoptosis (BAX, BID, Bcl-2, APAF, CYCS) and oxidative stress (SOD1, SOD2, CAT). Overall, C. calcitrans extracts demonstrated effective intervention against HepG2 cancer cells where enhanced apoptotic activities were observed with increased fucoxanthin content. (c) 2018 Published by Elsevier B.V.