Mapping of antigenic sites involved in serotype-cross-reactive neutralization on group A rotavirus outercapsid glycoprotein VP7.
Mapping of antigenic sites involved in serotype-cross-reactive neutralization on group A rotavirus outercapsid glycoprotein VP7.
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A 组轮状病毒外衣壳糖蛋白 VP7 上参与血清型交叉反应中和的抗原位点图谱。
DOI：
10.1006/viro.1994.1117
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发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
M. Gorziglia
中科院分区：
文献类型：
--
作者：
Y. Hoshino;K. Nishikawa;D. Benfield;M. Gorziglia
利用两种中和性单克隆抗体（n - mab）定位A组轮状病毒VP7上参与血清型交叉反应表位形成的氨基酸（aa）残基。N-mAb 954/159/13能中和G血清3型和猪G血清4型轮状病毒，而N-mAb 57/8能中和G血清3、4、6、9和10型轮状病毒。在这两种单克隆抗体存在的情况下，选择每种血清型的中和抗性变体。对这些变异的VP7编码基因序列分析表明：(1)变异区VR-5 （aa 88-100）、VR-8 （aa 209-223）和VR-9 （aa 235-242）参与交叉反应中和；（ii）由给定的N-mAb选择的不同血清型VP7上的相同位置可以发生aa取代；（iii）由给定N-mAb选择的变异VP7上aa取代的位置可能因轮状病毒血清型而异；（iv）单个N-mAb选择的VP7突变体上特定位置的单个替代aa种可能发生变化，导致突变体表现出抗原性差异；猪轮状病毒Gottfried株的VP7具有独特的血清型3和血清型4抗原嵌合体。
Two neutralizing monoclonal antibodies (N-mAbs) were utilized to locate amino acid (aa) residues involved in the formation of serotype-cross-reactive epitopes on the VP7 of selected group A rotaviruses. N-mAb 954/159/13 neutralized G serotype 3 as well as porcine G serotype 4 rotaviruses, whereas N-mAb 57/8 neutralized G serotype 3, 4, 6, 9, and 10 strains. Neutralization-resistant variants of each serotype were selected in the presence of these two monoclonal antibodies. Sequence analysis of the gene encoding VP7 of such variants revealed: (i) variable regions VR-5 (aa 88-100), VR-8 (aa 209-223), and VR-9 (aa 235-242) are involved in cross-reactive neutralization; (ii) an aa substitution can occur at the same position on the VP7 of different serotypes selected by a given N-mAb; (iii) the location of an aa substitution on the variant VP7 selected by a given N-mAb can vary depending on the rotavirus serotype; (iv) a substituted single aa species at a specific position on the variant VP7 selected by a single N-mAb can vary, resulting in variants which exhibit antigenic differences; and (v) the VP7 of a porcine rotavirus Gottfried strain has a unique antigenic mosaic of serotype 3 and serotype 4.