Multi-Omics Analysis on Neurodevelopment in Preterm Neonates: A Protocol Paper.
Multi-Omics Analysis on Neurodevelopment in Preterm Neonates: A Protocol Paper.
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DOI：
10.1097/nnr.0000000000000548
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发表时间：
2021-11-01
期刊：
影响因子：
2.5
通讯作者：
Cong XS
中科院分区：
文献类型：
--
作者：
Casavant SG;Chen J;Xu W;Lainwala S;Matson A;Chen MH;Starkweather A;Maas K;Cong XS
肠道微生物组是早产儿健康和疾病的重要决定因素。本文的目的是与其他新生儿重症监护病房分享我们目前的协议，以潜在地扩展其现有协议，旨在表征早产儿肠道微生物组与健康结局之间的关系。这项前瞻性纵向研究计划招募160名出生时胎龄< 32周或体重< 1,500克的早产儿，并入住两级III/IV新生儿重症监护病房之一。在新生儿重症监护室期间，主要指标包括生命早期疼痛/压力事件、肠道微生物组、宿主遗传变异和神经行为评估。在随访期间，测量了4个月、8-12个月和18-24个月校正年龄（CA）的肠道微生物组、疼痛敏感性以及医疗、生长和发育结果。我们假设，经历更高水平疼痛/压力的婴儿将改变肠道微生物组，包括潜在的不良后果，如坏死性小肠结肠炎（NEC）和宿主遗传变异，喂养不耐受和/或神经发育障碍。这些将不同于未发生这些不良后果的早产儿的肠道微生物组。为了验证这一假设，我们将确定肠道微生物组的改变如何影响早产儿发生NEC，喂养不耐受和神经发育障碍的风险。此外，我们还将研究肠道微生物组和宿主遗传学在肠道健康和神经发育结果调节中的相互作用。
The gut microbiome is an important determinant of health and disease in preterm infants. The objective of this paper is to share our current protocol for other neonatal intensive care units to potentially expand their existing protocols aiming to characterize the relationship between the intestinal microbiome and health outcomes in preterm infants. This prospective, longitudinal study planned to recruit 160 preterm infants born < 32 weeks’ gestational age or weighing < 1,500 grams and admitted to one of two-level III/IV neonatal intensive care units. During the neonatal intensive care unit period, the primary measures included events of early life pain/stress, gut microbiome, host genetic variations, and neurobehavioral assessment. During follow-up visits, gut microbiome, pain sensitivity, and medical, growth, and developmental outcomes at 4-, 8–12-, and 18–24-month corrected age (CA) were measured As of February 14, 2020, 214 preterm infants have been recruited. We hypothesize that infants who experience greater levels of pain/stress will have altered gut microbiome, including potential adverse outcomes such as necrotizing enterocolitis (NEC) and host genetic variations, feeding intolerance, and/or neurodevelopmental impairments. These will differ from the intestinal microbiome of preterm infants that do not develop these adverse outcomes. To test this hypothesis, we will determine how alterations in the intestinal microbiome affect the risk of developing NEC, feeding intolerance, and neurodevelopmental impairments in preterm infants. In addition, we will examine the interaction between the intestinal microbiome and host genetics in the regulation of intestinal health and neurodevelopmental outcomes.