Localization of dolichol in the lysosomal fraction of rat liver.
Localization of dolichol in the lysosomal fraction of rat liver.
复制标题
多醇在大鼠肝脏溶酶体部分中的定位。
DOI：
10.1016/s0021-9258(20)65187-0
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
W. Lennarz
中科院分区：
文献类型：
--
作者：
T. Wong;G. Decker;W. Lennarz
已研究了从大鼠肝匀浆制备的亚细胞级分中长萜醇和/或长萜醇酯的分布。皂化后的各个馏分的多萜醇分离和定量的高效液相色谱法在三个系统。通过标记酶和电子显微镜检查亚细胞制剂的纯度。使用差速离心，它被发现，长萜醇的水平是最高的在大肠杆菌-溶酶体馏分。在通过蔗糖密度离心进一步解析这一级分后，发现大部分的多萜醇与富含溶酶体的级分相关。相比之下，线粒体组分仅具有低水平的长萜醇。这一新的观察结果得到了证实，发现多萜醇大大富集在高度纯化的溶酶体部分制备分离Metrizamide密度离心。该纯化制剂中多萜醇的富集证实了该级分中溶酶体酶活性的观察到的富集。所有这些数据表明，大多数细胞长萜醇和/或长萜醇酯定位于溶酶体部分中。这一发现的意义有关的代谢的长萜醇进行了讨论。
The distribution of dolichol and/or dolichol esters in subcellular fractions prepared from a rat liver homogenate has been investigated. After saponification of the various fractions dolichol was isolated and quantitated by high performance liquid chromatography in three systems. The degree of purity of the subcellular preparations was examined by marker enzymes and by electron microscopy. Using differential centrifugation it was found that the level of dolichol was highest in the mitochondria-lysosome fraction. Upon further resolution of this fraction by sucrose density centrifugation it was found that the majority of the dolichol was associated with the lysosome-rich fraction. In contrast, the mitochondrial fraction had only a low level of dolichol. This novel observation was confirmed by the finding that dolichol was greatly enriched in a highly purified lysosome fraction preparations isolated by Metrizamide density centrifugation. The enrichment of dolichol in this purified preparation paralleled the observed enrichment of the lysosomal enzyme activity in this fraction. All of these data suggest that the majority of cellular dolichol and/or dolichol esters is localized in the lysosome fraction. The significance of this finding in relation to the metabolism of dolichol is discussed.
大鼠睾丸和附睾中的多醇浓度和生物合成。
影响因子：
3.6
作者：
Wenstrom,JC;Hamilton,DW
通讯作者：
Hamilton,DW
鸡胚胎发育过程中多醇和胆固醇的生物合成。
DOI：
10.1016/0005-2760(82)90186-2
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Wong,TK;Lennarz,WJ
通讯作者：
Lennarz,WJ
DOI：
10.1016/0005-2760(81)90074-6
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Adrian,GS;Keenan,RW
通讯作者：
Keenan,RW