Differences exist between viral transcripts in cottontail rabbit papillomavirus-induced benign and malignant tumors as well as non-virus-producing and virus-producing tumors.
Differences exist between viral transcripts in cottontail rabbit papillomavirus-induced benign and malignant tumors as well as non-virus-producing and virus-producing tumors.
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棉尾兔乳头瘤病毒诱导的良性和恶性肿瘤以及不产生病毒和产生病毒的肿瘤中的病毒转录本存在差异。
DOI：
10.1128/jvi.51.3.706-712.1984
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发表时间：
1984
影响因子：
5.4
通讯作者：
Wettstein,FO
中科院分区：
文献类型：
--
作者：
Nasseri,M;Wettstein,FO
在棉尾兔（该病毒的天然宿主）的产病毒肿瘤中发现了5种大小分别为4.8、2.6、2.0、1.3和0.9千碱基的棉尾兔乳头瘤病毒特异性聚腺苷化RNA。其中两种RNA（大小分别为2.0 kb和1.3 kb）在大小和图谱位置上与以前在非病毒产生的良性和恶性肿瘤中检测到的RNA无法区分（Nasseri et al.， J. Virol. 44:263-268, 1982）。在产生病毒的良性肿瘤中，2.0 kb RNA比1.3 kb RNA更丰富。这与对良性非病毒产生肿瘤的类似观察结果一起表明，2.0 kb RNA的优势是良性肿瘤的普遍特征。向优先合成1.3 kb RNA的转变似乎是肿瘤从乳头状瘤向癌进展的一种现象。三个转录本分别为4.8、2.6和0.9 kb，是产生病毒的肿瘤所特有的。RNA分子分两步绘制。首先，将Northern blots与亚基因组探针杂交，揭示了转录本的大致图谱位置。其次，利用核酸酶S1和外切酶VII作图程序和末端标记探针，精确定位了4.8-、2.6-、2.0-和1.3-kb rna的主要外显子，结果表明，所有rna都是从同一条DNA链转录而来。1.3 kb和2.0 kb的rna都由两个外显子组成，这两个外显子由一个相同的2.45 kb内含子分开。2.0 kb和1.3 kb rna的5‘-近端外显子的5’端分别映射到位置0.07和0.16。一些2.0 kb RNA分子，特别是在癌中，在0.06位置有一个替代的5'端。两个外显子的3'端映射到位置0.22，其中两个端相距约7个核苷酸。2.0 kb和1.3 kb rna的5'-近端外显子的大小分别为1.23和0.48 kb。1.3 kb和2.0 kb的rna共享一个共同的3'-近端外显子，长度为0.66 (0.61)kb。该外显子在图谱位置0.53处有两个5‘端，相距50个核苷酸，在图谱位置0.61处有一个3’端。只有4.8 kb和2.6 kb rna的3'-近端部分被精确定位。这两个rna在0.99位置有一个共同的3'端。2.6 kb的RNA部分由一个1.59 kb的外显子组成，延伸到图谱位置0.79。（摘要删节为400字）
Five major cottontail rabbit papillomavirus-specific polyadenylated RNA species with sizes of 4.8, 2.6, 2.0, 1.3, and 0.9 kilobases (kb) were found in virus-producing tumors of cottontail rabbits (the natural host for the virus). Two of the RNA species (sizes, 2.0 and 1.3 kb) are indistinguishable with respect to size and map position from the RNA species detected previously in non-virus-producing benign and malignant tumors (Nasseri et al., J. Virol. 44:263-268, 1982). The 2.0-kb RNA in virus-producing benign tumors is more abundant than the 1.3-kb RNA. This, together with similar observations of benign non-virus-producing tumors, suggests that the predominance of the 2.0-kb RNA is a general feature of benign tumors. The change to a preferential synthesis of the 1.3-kb RNA appears to be a phenomenon of tumor progression from papillomas to carcinomas. Three transcripts of 4.8, 2.6, and 0.9 kb are unique to virus-producing tumors. The RNA molecules were mapped in two steps. First, hybridization of Northern blots with subgenomic probes revealed the approximate map position of the transcripts. Second, with nuclease S1 and exonuclease VII mapping procedures and end-labeled probes, the major exons of the 4.8-, 2.6-, 2.0-, and 1.3-kb RNAs were mapped precisely, and it is shown that all RNAs are transcribed from the same DNA strand. Both 1.3- and 2.0- kb RNAs consist of two exons which are separated by an identical 2.45-kb intron. The 5' ends of the 5'-proximal exons of the 2.0- and 1.3-kb RNAs map to positions 0.07 and 0.16, respectively. Some of the 2.0-kb RNA molecules, especially in the carcinoma, have an alternative 5' end at position 0.06. The 3' ends of both exons map to position 0.22, where two ends were found about seven nucleotides apart. The sizes of the 5'-proximal exons of the 2.0- and 1.3-kb RNAs are 1.23 and 0.48 kb, respectively. The 1.3- and 2.0-kb RNAs share a common 3'-proximal exon of 0.66 (0.61) kb. This exon has two 5' ends 50 nucleotides apart at map position 0.53 and a 3' end at map position 0.61. Only the 3'-proximal part of the 4.8- and 2.6-kb RNAs have been mapped precisely. Both RNAs share a common 3' end at position 0.99. The 2.6-kb RNA part consists of a single 1.59-kb exon which extends to map position 0.79.(ABSTRACT TRUNCATED AT 400 WORDS)