Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials
Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials
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DOI：
10.4012/dmj.2009-082
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发表时间：
2010-05-01
影响因子：
2.5
通讯作者：
Nakano, Takayoshi
中科院分区：
文献类型：
--
作者：
Imazato, Satoshi;Horikawa, Daisuke;Nakano, Takayoshi
本研究使用一种典型的多能间充质前体细胞系C2C12，研究了多能间充质细胞在三种树脂基修复体上的增殖和分化能力。C2C12细胞在Bis-GMA/TEGDMA复合树脂(APX；Clearfi AP-X)、4-META/MMA树脂水泥(SB；Superond C&B)或含HEMA树脂修饰的玻璃离子材料(LC；富士离子II LC)的固化标本上培养3-21天。测定不同材料上培养细胞的碱性磷酸酶(ALP)活性，观察不同材料对细胞分化潜能的影响。在APX和SB上，细胞黏附和增殖良好，对ALP活性无明显影响。相反，在LC上培养的细胞增殖较差，ALP活性明显受到抑制，与以氧化锌EBA粘固剂为对照材料培养的细胞相似。与HEMA改性玻璃离子相比，BIS-GMA/TEGDMA复合树脂和4-META/MMA复合树脂对C2C12细胞具有更好的生物相容性，提示前两种树脂对根尖周或牙周组织再生的影响较小。
This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.