Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging
Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging
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DOI：
10.1364/boe.9.001492
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发表时间：
2018-04-01
影响因子：
3.4
通讯作者：
Dicarlo, James J.
中科院分区：
文献类型：
--
作者：
Ohayon, Shay;Caravaca-Aguirre, Antonio;Dicarlo, James J.
神经科学中的一个主要的开放性挑战是能够在大脑中任何地方以细胞分辨率测量和干扰来自明确定义的神经亚群的体内神经活动。然而，散射和吸收造成的限制禁止用于深（> 2 mm）结构的非侵入性多光子方法，而梯度折射率（GRIN）内窥镜相对较厚，并且在插入时可能导致显著损伤。在这里，我们提出了一种新型的微内窥镜设计，通过超薄多模光纤（MMF）探头，具有5- 10倍细的直径比市售的显微内窥镜在任意深度的神经活动的图像。我们展示了微米级分辨率，多光谱和体积成像。与以前的方法相比，我们表明，这种方法具有提高的采集速度，足以捕捉快速的神经元动力学在啮齿动物体内表达的遗传编码的钙指示剂（GCaMP）。我们的研究结果强调了这项技术在神经科学应用中的潜力，并为以前无法到达的大脑区域的细胞分辨率成像开辟了可能性。(c)根据OSA开放获取出版协议的条款，2018年美国光学学会
A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (> 2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available microendoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement