POLYMER SUPPORTED DNA-SYNTHESIS USING HYDROXYBENZOTRIAZOLE ACTIVATED PHOSPHOTRIESTER INTERMEDIATES
POLYMER SUPPORTED DNA-SYNTHESIS USING HYDROXYBENZOTRIAZOLE ACTIVATED PHOSPHOTRIESTER INTERMEDIATES
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DOI：
10.1093/nar/12.22.8639
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
VANBOOM, JH
中科院分区：
文献类型：
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作者：
MARUGG, JE;PIEL, N;VANBOOM, JH
N-酰基-5‘-0-DMTR-d-核苷或类似保护的具有自由3’-0H基团的DNA-二聚体与2-氯苯基-0，0-二(1-苯并三唑)磷酸反应得到活性3‘-磷酸三酯衍生物。后者无需进一步提纯即可用于在可控孔玻璃/长链烷基胺载体上合成DNA片段。此外，2-氰基-1，1-二甲基乙氧基二氯膦非常适合于在相同类型的固体载体上制备5‘-磷酸化DNA片段。
Phosphorylation of N-acyl-5′-0-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3′-0H group, with 2-chlorophenyl-0, 0-bis(1-benzotriazolyl)phosphate affords reactive 3′-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesis of DNA-fragments on the controlled pore glass/long chain alkylamine support. Further, 2-cyano-1, 1-dimethylethoxy dichlorophosphine proved to be very suitable for the preparation of 5′-phosphorylated DNA-fragments on the same type of solid support.