Genetically highly divergent RNA virus with astrovirus-like (5'-end) and hepevirus-like (3'-end) genome organization in carnivorous birds, European roller (Coracias garrulus).
Genetically highly divergent RNA virus with astrovirus-like (5'-end) and hepevirus-like (3'-end) genome organization in carnivorous birds, European roller (Coracias garrulus).
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DOI：
10.1016/j.meegid.2019.04.003
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发表时间：
2019-07
期刊：
影响因子：
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通讯作者：
P. Pankovics;Á. Boros;T. Kiss;P. Engelmann;G. Reuter
中科院分区：
文献类型：
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作者：
P. Pankovics;Á. Boros;T. Kiss;P. Engelmann;G. Reuter
星形病毒（星形病毒科）和肝炎病毒（肝炎病毒科）是小型的无包膜病毒，具有遗传多样性+ssRNA基因组，被认为是感染包括人类在内的脊椎动物的肠道病原体。最近，许多新的天文和戊型肝炎病毒样+ssRNA病毒已被描述从低等脊椎动物物种。宇宙病毒和肝炎病毒的非结构蛋白高度多样化，但结构/衣壳蛋白代表一个共同的系统发育位置，揭示了它们通过病毒间重组的共同起源。本研究利用RT-PCR技术，从匈牙利35份欧洲卷耳猴（Coracias garrulus）粪便样本中的3份（8.5%）中，偶然发现并鉴定了一种具有+ssRNA基因组的新型星状病毒/肝炎病毒样病毒（Er/SZAL5/HUN/2011, MK450332）。Er/SZAL5/HUN/2011 （MK450332）的全基因组长度为8402 nt- t，可能由三个不重叠的开放阅读框（orf）组成：ORF1a(4449 nt/1482aa), ORF1b（1206 nt/401aa）和ORF2（1491 nt/496aa）。ORF1ab具有类似星状病毒的基因组组织，包含非结构保守元件（TM、CC、NLS、VPg）和酶残基（胰蛋白酶样蛋白酶、rna依赖rna聚合酶），氨基酸序列与星状病毒的同源性较低，分别为15% （ORF1a）和44% （ORF1b）。据推测ORF2是一种衣壳蛋白，但无论是星状病毒样亚基因组RNA启动子（sgRNA）还是星状病毒样衣壳特征都没有被识别出来。然而，预测的衣壳蛋白（ORF2）与类似hepevirus的新型Rana hepevirus （MH330682）对应蛋白的同源性为26%。这种新的+ssRNA病毒株Er/SZAL5/HUN/2011在非结构基因组区域（ORF1a和ORF1b）和Rana hepevirus相关衣壳（ORF2）蛋白具有类似星状病毒的基因组组织，代表了一种潜在的重组病毒物种，并支持共同起源假说，尽管所研究病毒的分类位置仍在讨论中。
Astroviruses (familyAstroviridae) and hepeviruses (family Hepeviridae) are small, non-enveloped viruses with genetically diverse +ssRNA genome thought to be enteric pathogens infecting vertebrates including humans. Recently, many novel astro- and hepatitis E virus-like +ssRNA viruses have been described from lower vertebrate species. The non-structural proteins of astro- and hepeviruses are highly diverse, but the structural/capsid proteins represent a common phylogenetic position shed the light of their common origin by inter-viral recombination. In this study, a novel astrovirus/hepevirus-like virus with +ssRNA genome (Er/SZAL5/HUN/2011, MK450332) was serendipitously identified and characterized from 3 (8.5%) out of 35 European roller (Coracias garrulus) faecal samples by RT-PCR in Hungary. The complete genome of Er/SZAL5/HUN/2011 (MK450332) is 8402 nt-long and potentially composed three non-overlapping open reading frames (ORFs): ORF1a (4449 nt/1482aa), ORF1b (1206 nt/401aa) and ORF2 (1491 nt/496aa). The ORF1ab has an astrovirus-like genome organization containing the non-structural conserved elements (TM, CC, NLS, VPg) and enzyme residues (trypsine-like protease, RNA-dependent RNA-polymerase) with low amino acid sequence identity, 15% (ORF1a) and 44% (ORF1b), to astroviruses. Supposedly the ORF2 is a capsid protein but neither the astrovirus-like subgenomic RNA promoter (sgRNA) nor the astrovirus-like capsid characteristics have been identifiable. However, the predicted capsid protein (ORF2) showed 26% identity to the corresponding protein of hepevirus-like novel Rana hepevirus (MH330682). This novel +ssRNA virus strain Er/SZAL5/HUN/2011 with astrovirus-like genome organization in the non-structural genome regions (ORF1a and ORF1b) and Rana hepevirus-related capsid (ORF2) protein represent a potentially recombinant virus species and supports the common origin hypothesis, although, the taxonomic position of the studied virus is still under discussion.