SPERM MOTILITY INHIBITOR FROM HUMAN SEMINAL PLASMA - ASSOCIATION WITH SEMEN COAGULUM
SPERM MOTILITY INHIBITOR FROM HUMAN SEMINAL PLASMA - ASSOCIATION WITH SEMEN COAGULUM
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DOI：
10.1093/oxfordjournals.humrep.a136267
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发表时间：
1995-08-01
影响因子：
6.1
通讯作者：
GAGNON, C
中科院分区：
文献类型：
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作者：
ROBERT, M;GAGNON, C
人的精浆中含有一种精子运动抑制物(SPMI)，它来源于精囊，是一种52 kDa的前体形式，射精后可被前列腺酶迅速降解。在本研究中，分析了SPMI生物活性和抗原在化学诱导和自然发生的精液液化停止中的分布。SPMI活性仅在2200+/-560IU的凝固精液部分中检测到，而精浆总蛋白在可溶组分和凝固组分之间更均匀地分离(分别为91+/-19和65+/-18 mg)。SPMI抗血清识别凝固体中52、38、35、33和20 kDa不同形式的SPMI前体，而可溶性蛋白组分在15 kDa处只有一条主要免疫反应带。在室温下不能自发液化的精液样本中，检测到高水平的SPMI活性(1500+/-180IU/ml)、高分子形式的SPMI前体和低精子活动率(26%)。在未液化的样品中加入前列腺液，导致SPMI活性下降(330+/-17IU/ml)，并将SPMI前体转变为低分子形式(14-22 kDa)，同时精子活动率增加到49%。结果表明，SPMI与精液凝固物成分高度相关，因为它们是非常活跃的形式，如果在射精后处理不当，可能会对精子的活力产生不利影响。
Human seminal plasma contains a sperm motility inhibitor (SPMI) originating from the seminal vesicles as a 52 kDa precursor form that is rapidly degraded by prostatic proteases after ejaculation. In this study, the distribution of SPMI biological activity and antigens was analysed in chemically induced, as well as naturally occurring, arrest of semen liquefaction. SPMI activity was detected exclusively in the coagulated semen fraction at 2200 +/- 560 IU, whereas total seminal plasma proteins separated more evenly between soluble and coagulated components (91 +/- 19 and 65 +/- 18 mg, respectively). An SPMI antiserum recognized different forms of SPMI precursors at 52, 38, 35, 33 and 20 kDa in the coagulum while the soluble protein fraction contained only one major immunoreactive band at 15 kDa. High levels of SPMI activity (1500 +/- 180 IU/ml) together with high molecular mass forms of SPMI precursor and low sperm motility (26%) were detected in semen samples that failed to liquefy spontaneously at room temperature. Addition of prostatic secretions to the non-liquefying samples caused a decrease of SPMI activity (330 +/- 17 IU/ml) and transformed the SPMI precursor into low molecular mass forms (14-22 kDa) with a concomitant increase in sperm motility to 49%. The results suggest that SPMI is highly associated with the seminal coagulum components as very active forms that may adversely affect sperm motility when not properly processed after ejaculation.