Ascorbic Acid, Inflammatory Cytokines (IL-1β/TNF-α/IFN-γ), or Their Combination's Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal Stem/Progenitor Cells
Ascorbic Acid, Inflammatory Cytokines (IL-1β/TNF-α/IFN-γ), or Their Combination's Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal Stem/Progenitor Cells
复制标题
DOI：
10.1155/2020/8897138
复制
发表时间：
2020-08-17
影响因子：
4.3
通讯作者：
Doerfer, Christof E.
中科院分区：
文献类型：
--
作者：
Fawzy El-Sayed, Karim M.;Nguyen, Nhung;Doerfer, Christof E.
客观的。抗坏血酸（AA）和受控炎症刺激被认为能够独立对牙龈间充质干细胞/祖细胞（G-MSC）的各种增殖、多能性和分化属性产生积极影响。本研究的目的是首次探讨和比较主要炎症细胞因子（IL-1β/TNF-α/IFN-γ）、AA或其组合对G-MSCs的多能性/多能性、增殖和分化特征的影响。分离人G-MSCs（n=5）并在基础培养基（对照组）中培养，在含有主要炎症细胞因子的基础培养基中； 1 ng/ml IL-1 beta、10 ng/ml TNF-α和100 ng/ml IFN-gamma（炎症组），含250 μmol/l AA的基础培养基（AA组）和补充AA的炎症培养基（炎症/AA组）。所有介质每周更新三次。在刺激的 G-MSC 中，检测 1 小时的细胞内 β-连环蛋白、第 1、3 和 5 天的多能性基因表达，以及 14 天的集落形成单位 (CFU) 能力和细胞增殖。在指定组中进行五天刺激后，通过定性和定量组织化学以及 mRNA 表达来评估多谱系分化。结果：所有实验组中β-连环蛋白的细胞内显着减少（p = 0.002，Friedman）。 AA 组在第 3、6、7 和 13 天表现出显着更高的细胞计数 (p
Objective. Ascorbic acid (AA) and controlled inflammatory stimuli are postulated to possess the ability to independently exert positive effects on a variety of proliferative, pluripotency, and differentiation attributes of gingival mesenchymal stem/progenitor cells (G-MSCs). The current study's objective was to explore and compare for the first time the impact of the major inflammatory cytokines (IL-1 beta/TNF-alpha/IFN-gamma), AA, or their combination on multipotency/pluripotency, proliferative, and differentiation characteristics of G-MSCs.Design. Human G-MSCs (n=5) were isolated and cultured in basic medium (control group), in basic medium with major inflammatory cytokines; 1 ng/ml IL-1 beta, 10 ng/ml TNF-alpha, and 100 ng/ml IFN-gamma(inflammatory group), in basic medium with 250 mu mol/l AA (AA group) and in inflammatory medium supplemented by AA (inflammatory/AA group). All media were renewed three times per week. In stimulated G-MSCs intracellular beta-catenin at 1 hour, pluripotency gene expression at 1, 3, and 5 days, as well as colony-forming units (CFUs) ability and cellular proliferation over 14 days were examined. Following a five-days stimulation in the designated groups, multilineage differentiation was assessed via qualitative and quantitative histochemistry as well as mRNA expression.Results.beta-Catenin significantly decreased intracellularly in all experimental groups (p=0.002, Friedman). AA group exhibited significantly higher cellular counts on days 3, 6, 7, and 13 (p