TRANS ACTIVATION OF TRANSCRIPTION BY HERPES-VIRUS PRODUCTS - REQUIREMENT FOR 2 HSV-1 IMMEDIATE-EARLY POLYPEPTIDES FOR MAXIMUM ACTIVITY
TRANS ACTIVATION OF TRANSCRIPTION BY HERPES-VIRUS PRODUCTS - REQUIREMENT FOR 2 HSV-1 IMMEDIATE-EARLY POLYPEPTIDES FOR MAXIMUM ACTIVITY
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DOI：
10.1002/j.1460-2075.1984.tb02270.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
EVERETT, RD
中科院分区：
文献类型：
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作者：
EVERETT, RD
疱疹病毒的转录程序可分为3个主要阶段。立即早期（IE）基因首先被预先存在的宿主RNA聚合酶II转录，它们的启动子受到病毒颗粒多肽成分的强烈刺激。E和L基因启动子只有在IE基因产物出现后才具有活性。遗传和生化证据表明，单纯疱疹病毒1型IE多肽Vmw175 （ICP 4）对HSV早期启动子的反式激活至关重要，但其他4种IE基因产物的作用尚不清楚。本研究描述了通过将重组质粒共转染HeLa细胞的功能试验表明，Vmw175可以单独激活一个HSV-1 E基因启动子，其他4个HSV-1 IE基因产物本身不能激活转录，Vmw175与IE基因1产物Vmw110 （ICP 0）的组合比Vmw175单独激活的效果更好，克隆了人巨细胞病毒（CMV） IE基因产物。水痘带状疱疹病毒（VZV）和伪狂犬病毒（PRV）也可以激活HSV-1早期启动子的转录，这种激活也发生在细胞启动子上。
The transcriptional program of the herpesviruses is organized into 3 prinicipal phases. The immediate-early (IE) genes are the first to be transcribed, by the pre-existing host RNA polymerase II and their promoters are strongly stimulated by a polypeptide component of the virus particle. The E and L gene promoters become active only after the appearance of IE gene products. Genetic and biochemical evidence has shown that the HSV-1 [herpes simplex virus type 1] IE polypeptide Vmw175 (ICP 4) is essential for the trans activation of HSV early promoters, but the role of none of the other 4 IE gene products was known. This study describes functional tests that show, by co-transfection of recombinant plasmids into HeLa cells, that Vmw175 alone can activate an HSV-1 E gene promoter, the 4 other HSV-1 IE gene products by themselves are unable to activate transcription, the combination of Vmw175 plus the product of IE gene 1, Vmw110 (ICP 0), is a much better activator than Vmw175 alone, cloned IE gene products of human cytomegalovirus (CMV), varicella-zoster virus (VZV) and pseudorabies virus (PRV) can also activate transcription from an HSV-1 early promoter and this activation also occurs with cellular promoters.