Comparison of Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Platforms for the Identification of Gram-Negative Rods from Patients with Cystic Fibrosis
Comparison of Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Platforms for the Identification of Gram-Negative Rods from Patients with Cystic Fibrosis
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DOI：
10.1128/jcm.01618-13
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发表时间：
2013-11-01
影响因子：
9.4
通讯作者：
Miller, Melissa B.
中科院分区：
文献类型：
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作者：
Alby, Kevin;Gilligan, Peter H.;Miller, Melissa B.
我们使用SARAMIS v4.09和Knowledge Base v2.0数据库对Bruker Biotyper和bioMerieux Vitek MS进行了性能评估，以鉴定203种非葡萄糖发酵革兰氏阴性棒，这些革兰氏阴性棒先前已通过16S rRNA基因测序鉴定。包括重复检测在内，分别有96.6%、90.1%和93.6%的分离株鉴定与先前鉴定一致。
We evaluated the performance of the Bruker Biotyper and the bioMerieux Vitek MS with both the SARAMIS v4.09 and Knowledge Base v2.0 databases for the identification of 203 non-glucose-fermenting Gram-negative rods that had previously been identified by 16S rRNA gene sequencing. Including those that underwent repeat testing, 96.6%, 90.1%, and 93.6% of isolates, respectively, had identifications that agreed with the previous identification.