First report of foregut microbial community in proboscis monkeys: are diverse forests a reservoir for diverse microbiomes?
First report of foregut microbial community in proboscis monkeys: are diverse forests a reservoir for diverse microbiomes?
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DOI：
10.1111/1758-2229.12677
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发表时间：
2018-12-01
影响因子：
3.3
通讯作者：
Matsuda, Ikki
中科院分区：
文献类型：
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作者：
Hayakawa, Takashi;Nathan, Senthilvel K. S. S.;Matsuda, Ikki
众所周知，前肠发酵发生在广泛的哺乳动物物种和单一的鸟类物种中。然而，自由放养的前肠发酵猴的前肠微生物群落，即colobines，迄今尚未进行过研究。我们分析了前肠微生物组在四个自由放养长鼻猴（Nasalis larvatus）从两个不同的热带栖息地与不同的植物多样性（红树林和河边森林），在一个人从一个半自由放养的设置与补充饲料，并在一个人从圈养，使用高通量测序的基础上16 S核糖体RNA基因。我们发现，减少前肠微生物多样性从一个多样化的自然栖息地（河边森林），一个低多样性的自然栖息地（红树林），人类相关的环境。在所有环境中检测到的总共2700个细菌操作分类单位（OTU）中，只有153个OTU在所有个体中共享，这表明它们不受饮食或栖息地的影响。这些OTU由厚壁菌门和变形菌门占主导地位。栖息地特定的微生物群落的相对丰度显示出广泛的生活环境之间的差异，虽然这样的细菌群落似乎是由厚壁菌门和拟杆菌门占主导地位，这表明这些门是关键的理解在长鼻猴生活在不同的栖息地的适应性策略。
Foregut fermentation is well known to occur in a wide range of mammalian species and in a single bird species. Yet, the foregut microbial community of free-ranging, foregut-fermenting monkeys, that is, colobines, has not been investigated so far. We analysed the foregut microbiomes in four free-ranging proboscis monkeys (Nasalis larvatus) from two different tropical habitats with varying plant diversity (mangrove and riverine forests), in an individual from a semi-free-ranging setting with supplemental feeding, and in an individual from captivity, using high-throughput sequencing based on 16S ribosomal RNA genes. We found a decrease in foregut microbial diversity from a diverse natural habitat (riverine forest) to a low diverse natural habitat (mangrove forest), to human-related environments. Of a total of 2700 bacterial operational taxonomic units (OTUs) detected in all environments, only 153 OTUs were shared across all individuals, suggesting that they were not influenced by diet or habitat. These OTUs were dominated by Firmicutes and Proteobacteria. The relative abundance of the habitat-specific microbial communities showed a wide range of differences among living environments, although such bacterial communities appeared to be dominated by Firmicutes and Bacteroidetes, suggesting that those phyla are key to understanding the adaptive strategy in proboscis monkeys living in different habitats.