A dual Purkinje cell rate and synchrony code sculpts reach kinematics.
A dual Purkinje cell rate and synchrony code sculpts reach kinematics.
复制标题
双浦肯野细胞速率和同步代码雕刻达到了运动学。
DOI：
10.1101/2023.07.12.548720
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Person,AbigailL
中科院分区：
文献类型：
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作者：
Nashef,Abdulraheem;Spindle,MichaelS;Calame,DylanJ;Person,AbigailL
小脑浦肯野细胞（PCs）在其种群放电速率中编码运动运动学。据推测，放电速率抑制可以解除对小脑核神经元的抑制，促进适应性运动调节。然而，关于第二种去抑制机制（PC简单尖峰同步）是否与种群编码相关的争论仍在继续。我们通过将高密度探针记录的PC率和同步模式与鼠标到达运动学相关联来解决这个问题。我们发现PC同步的行为相关性与小脑输出活动之间已知的因果关系一致。到达减速与浦肯野放电速率降低和同步正相关，这与两种机制解除抑制目标神经元一致，目标神经元调节到达速度。采用动态钳形法，结合生理速率和体外同步模式，直接测试了每种编码方案对核发射的贡献，证实了生理水平的PC简单尖峰同步对核发射具有高度促进作用。这些发现表明，PC放电速率和同步协同发挥精细的运动控制。
Cerebellar Purkinje cells (PCs) encode movement kinematics in their population firing rates. Firing rate suppression is hypothesized to disinhibit neurons in the cerebellar nuclei, promoting adaptive movement adjustments. Debates persist, however, about whether a second disinhibitory mechanism, PC simple spike synchrony, is a relevant population code. We addressed this question by relating PC rate and synchrony patterns recorded with high density probes, to mouse reach kinematics. We discovered behavioral correlates of PC synchrony that align with a known causal relationship between activity in cerebellar output. Reach deceleration was positively correlated with both Purkinje firing rate decreases and synchrony, consistent with both mechanisms disinhibiting target neurons, which are known to adjust reach velocity. Direct tests of the contribution of each coding scheme to nuclear firing using dynamic clamp, combining physiological rate and synchrony patterns ex vivo, confirmed that physiological levels of PC simple spike synchrony are highly facilitatory for nuclear firing. These findings suggest that PC firing rate and synchrony collaborate to exert fine control of movement.