Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes.
Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes.
复制标题
使用细菌全细胞作为分子探针分离病原体特异性人单克隆抗体 (hmAb)。
DOI：
10.1007/978-1-0716-0795-4_2
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Siris S
中科院分区：
文献类型：
--
作者：
Siris S
The immunoglobulin capture assay (ICA) enables the enrichment for pathogen-specific plasmablasts from individuals with a confirmed adaptive immune response to vaccination or disseminated infection. Only single recombinant antigens have been used previously as probes in this ICA and it was unclear whether the method was applicable to complex probes such as whole bacterial cells. Here, we describe the enrichment of plasmablasts specific for polysaccharide and protein antigens of bothStreptococcus pneumoniaeandNeisseria meningitidisusing whole formalin-fixed bacterial cells as probes. The modified ICA protocol described here allowed for a pathogen-specific hmAb cloning efficiency of >80%.
影响因子：
14.8
作者：
Mazutis, Linas;Gilbert, John;Ung, W. Lloyd;Weitz, David A.;Griffiths, Andrew D.;Heyman, John A.
通讯作者：
Heyman, John A.
影响因子：
14.8
作者：
Lin, Zhonghua;Chiang, Nancy Y.;Swem, Lee R.
通讯作者：
Swem, Lee R.