Zygotic nanos3 Mutant Medaka (Oryzias latipes) Displays Gradual Loss of Germ Cells and Precocious Spermatogenesis During Gonadal Development
Zygotic nanos3 Mutant Medaka (Oryzias latipes) Displays Gradual Loss of Germ Cells and Precocious Spermatogenesis During Gonadal Development
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DOI：
10.2108/zs210123
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发表时间：
2022-03
影响因子：
0.9
通讯作者：
T. Nishimura;Minoru Tanaka
中科院分区：
文献类型：
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作者：
T. Nishimura;Minoru Tanaka
Nanos是种质的组成部分之一，从苍蝇到哺乳动物都是进化保守的。在青鳉（Oryzias latipes）中，母体提供的nanos 3对于维持原始生殖细胞（PGCs）至关重要。在这里，我们通过使用转录激活因子样效应核酸酶（TALEN）产生nanos 3功能丧失突变体，并研究了青鳉合子nanos 3的功能。来自nanos 3杂合母体的合子nanos 3纯合（-/-）突变体形成生殖细胞。然而，孵化后，生殖细胞的数量大大减少，导致nanos 3-/-雌性和雄性不育。令人惊讶的是，nanos 3-/- XX和XY突变体在早期性腺发育过程中发生了早熟精子发生，如在青鳉中foxl 3（生殖系性别决定基因）功能丧失突变体中所见。因此，除了生殖细胞的维护，这些结果表明，合子nanos 3影响XX青鳉生殖系性别的适当调节。
Nanos is one of the components of germ plasm and is evolutionarily conserved from flies to mammals. In medaka (Oryzias latipes), maternally provided nanos3 is essential for maintenance of primordial germ cells (PGCs). Here, we generated nanos3 loss-of-function mutants by using transcription activator-like effector nuclease (TALEN) and examined the function of zygotic nanos3 in medaka. Zygotic nanos3 homozygous (–/–) mutants derived from nanos3 heterozygous mothers formed germ cells. However, after hatching, the number of germ cells decreased considerably, resulting in infertility of both nanos3–/– females and males. Surprisingly, both nanos3–/– XX and XY mutants underwent precocious spermatogenesis during early gonadal development, as seen in loss-of-function mutants of foxl3, the germline sex-determination gene, in medaka. Therefore, in addition to the maintenance of germ cells, these results suggest that zygotic nanos3 affects the proper regulation of germline sex in XX medaka.