Production of Monosex All-Male Nile Tilapia Using Ethanol Extract of Tribulus terrestris Seeds
Production of Monosex All-Male Nile Tilapia Using Ethanol Extract of Tribulus terrestris Seeds
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DOI：
10.1007/s12595-017-0254-7
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发表时间：
2017-11
影响因子：
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通讯作者：
Indranath Ghosal;S. Chakraborty
中科院分区：
文献类型：
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作者：
Indranath Ghosal;S. Chakraborty
尼罗罗非鱼混合性别幼鱼平均体重0.025 ± 0.009g;平均体长（1.25 ± 0.012 cm）（商品鱼饲料，日本东急，30%粗蛋白）与不同浓度的蒺藜种子的乙醇提取物（0.0、2.0、2.5、3.0 g/kg饲料）以评价植物种子提取物诱导鱼雄性化的功效。与对照相比，用不同浓度的植物提取物处理导致显著更高（P< 0.05）的存活百分比，表明植物提取物没有不利的健康影响。与喂食对照饮食的鱼相比，喂食含有植物提取物的饮食的鱼显示出显著更高（P< 0.05）的雄性百分比。饲料中添加2.0g/kg饲料的植物提取物的雄性百分率最高（91.53 ± 0.38），可以认为是单性别罗非鱼生产的最佳浓度。蒺藜提取物研究结果表明，木霉乙醇提取物具有较好的生物活性。terrestrisseeds可能具有诱导鱼类性别逆转的潜力，这种可生物降解的天然植物材料可用于生产单性别罗非鱼，而不是合成类固醇。
Mixed sex juveniles of Nile tilapia (mean weight 0.025 ± 0.009 g; mean length 1.25 ± 0.012 cm) were subjected to dietary treatment (commercial fish feed, Tokyu, Japan, 30% crude protein) with ethanolic extract ofTribulus terrestrisseeds at different concentrations (0.0, 2.0, 2.5, 3.0 g/kg feed) to evaluate the efficacy of the plant seed extract for induction of masculinisation in fish. Treatment with plant extract at different concentrations have resulted in significantly higher (P< 0.05) survival percentage compared to control indicating no adverse health effect of the plant extract. Fish fed diets containing the plant extract showed significantly higher (P< 0.05) percentage of males compared to fish fed control diet. Dietary supplementation with the plant extract at the concentration of 2.0 g/kg feed showed the highest percentage (91.53 ± 0.38) of males and may be considered to be the best concentration among the selected concentrations for monosex tilapia production withT. terrestrisseeds extract. The result of the study indicates that ethanolic extract ofT. terrestrisseeds may have potential for inducing sex reversal in fish and this biodegradable natural plant material may be used for monosex tilapia production instead of synthetic steroids.