Comparison of synthetic and natural glucosylceramides as substrate for glucosylceramidase assay.
Comparison of synthetic and natural glucosylceramides as substrate for glucosylceramidase assay.
复制标题
合成和天然葡萄糖神经酰胺作为葡萄糖神经酰胺酶测定底物的比较。
DOI：
10.1016/0009-8981(82)90221-2
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Suzuki,K
中科院分区：
文献类型：
--
作者：
Vaccaro,AM;Kobayashi,T;Suzuki,K
Commercially available [3H]glucosylceramide is derived from spleen tissue of patients with Gaucher's disease. When such tritiated glucosylceramide was diluted with unlabelled glucosylceramide from different sources and used as the substrate for assays of glucosylceramidase, the apparent activities obtained differed drastically. When diluted with syntheticN-stearoyl- orN-lignoceroyl-glucosyldihydrosphingosine, the release of [3H]glucose was 4–5 times greater than when diluted with unlabelled glucosylceramide from Gaucher spleen, with either brain or fibroblast homogenate as the enzyme source. TheKivalues of the synthetic substrates were 15–30 times greater than theKmfor the natural mixture, indicating much lower affinity of the synthetic substrates for the enzyme. Although the reason for the reduction in affinity could not be identified, caution is required when the commercial [3H]glucosylceramide is to be diluted with unlabelled glucosylceramide.
