Laser-assisted cell removing (LACR) technology contributes to the purification process of the undifferentiated cell fraction during pluripotent stem cell culture
Laser-assisted cell removing (LACR) technology contributes to the purification process of the undifferentiated cell fraction during pluripotent stem cell culture
复制标题
激光辅助细胞去除（LACR）技术有助于多能干细胞培养过程中未分化细胞部分的纯化过程
DOI：
10.1016/j.bbrc.2018.08.101
复制
发表时间：
2018
影响因子：
3.1
通讯作者：
Suemori Hirofumi
中科院分区：
文献类型：
--
作者：
Teramura Takeshi;Matsuda Koichi;Takehara Toshiyuki;Shinohara Kenji;Miyashita Yuji;Mieno Yasumichi;Mori Tatsufumi;Fukuda Kanji;Suzuki Koichi;Suemori Hirofumi
通过去除分化部分纯化未分化细胞是多能干细胞培养中的重要步骤。该过程传统上在显微镜下使用细玻璃毛细管或塑料尖端手动进行，或通过在补充有抗分化抑制剂的选择性培养基中培养来进行。然而，存在与这些方法相关的几个不可避免的问题，例如污染或生物副作用。在这里，我们开发了一种激光辅助细胞去除（LACR）技术，可以实现精确，快速和非接触式细胞去除。将LACR技术与计算机图像识别/鉴别技术相结合，实现了细胞自动纯化（A-LACR）。A-LACR的实用性通过两个证明进行评估：从人iPS和TS细胞共培养物中选择性去除滋养层干（TS）细胞，以及通过靶向自发发育的分化细胞纯化未分化的iPS细胞。我们的研究结果表明，LACR技术是再生医学中干细胞处理的一种新方法。
Purification of undifferentiated cells by removing differentiated parts is an essential step in pluripotent stem cell culture. This process has been traditionally performed manually using a fine glass capillary or plastic tip under a microscope, or by culturing in a selective medium supplemented with anti-differentiation inhibitors. However, there are several inevitable problems associated with these methods, such as contamination or biological side-effects. Here, we developed a laser-assisted cell removing (LACR) technology that enables precise, fast, and contact-less cell removal. Using LACR combined with computational image recognition/identification-discriminating technology, we achieved automatic cell purification (A-LACR). Practicability of A-LACR was evaluated by two demonstrations: selective removal of trophoblast stem (TS) cells from human iPS and TS cell co-cultures, and purification of undifferentiated iPS cells by targeting differentiated cells that spontaneously developed. Our results suggested that LACR technology is a novel approach for stem cell processing in regenerative medicine.