Memory-enhancing intra-basolateral amygdala infusions of clenbuterol increase Arc and CaMKIIα protein expression in the rostral anterior cingulate cortex
Memory-enhancing intra-basolateral amygdala infusions of clenbuterol increase Arc and CaMKIIα protein expression in the rostral anterior cingulate cortex
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DOI：
10.3389/fnbeh.2012.00017
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发表时间：
2012-04-19
影响因子：
3
通讯作者：
McIntyre, Christa K.
中科院分区：
文献类型：
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作者：
Holloway-Erickson, Crystal M.;McReynolds, Jayme R.;McIntyre, Christa K.
杏仁核基底外侧复合体（BLA）中β-肾上腺素受体的激活通过与多个记忆系统的相互作用来调节记忆。这种相互作用的细胞机制仍然没有得到解决。记忆调节BLA操作影响背侧海马中即刻早期基因活性调节的细胞凋亡相关蛋白（Arc）的蛋白产物的表达，并且Arc蛋白的海马表达与记忆巩固和长时程增强密切相关。目前的研究检查是否这种影响的BLA是特定的海马和弧蛋白。与海马一样，前扣带皮层（rACC）的吻侧部分参与抑制性回避（IA）记忆的巩固，IA训练增加rACC中的Arc蛋白。由于BLA与rACC在IA记忆的巩固中相互作用，rACC是进一步研究BLA调节突触可塑性的潜在候选者。钙/钙调蛋白依赖性蛋白激酶II（CaMKII α）的α亚型和立即早期基因c-Fos参与长时程增强和记忆。Arc和CaMKII α蛋白都可以在分离的突触中翻译，其中mRNA被定位，但c-Fos蛋白保留在索马中。为了研究BLA的记忆调节操作对rACC中这些记忆和可塑性相关蛋白表达的影响，对雄性Sprague-Dawley大鼠进行IA任务训练，并在训练后立即给予BLA内克伦特罗或利多卡因输注。研究结果表明，BLA的去甲肾上腺素能刺激可能通过对突触蛋白Arc和CaMKII α的影响来调节记忆巩固，但不是体细胞蛋白c-Fos。此外，BLA操作后在rACC中观察到的蛋白质变化表明，BLA对突触蛋白的影响不仅限于背侧海马中的那些。
Activation of beta-adrenoceptors in the basolateral complex of the amygdala (BLA) modulates memory through interactions with multiple memory systems. The cellular mechanisms for this interaction remain unresolved. Memory-modulating BLA manipulations influence expression of the protein product of the immediate early gene activity-regulated cytoskeletal-associated protein (Arc) in the dorsal hippocampus, and hippocampal expression of Arc protein is critically involved in memory consolidation and long-term potentiation. The present studies examined whether this influence of the BLA is specific to the hippocampus and to Arc protein. Like the hippocampus, the rostral portion of the anterior cingulate cortex (rACC) is involved in the consolidation of inhibitory avoidance (IA) memory, and IA training increases Arc protein in the rACC. Because the BLA interacts with the rACC in the consolidation of IA memory, the rACC is a potential candidate for further studies of BLA modulation of synaptic plasticity. The alpha isoform of the Calcium/Calmodulin-dependent protein kinase II (CaMKII alpha) and the immediate early gene c-Fos are involved in long-term potentiation and memory. Both Arc and CaMKII alpha proteins can be translated in isolated synapses, where the mRNA is localized, but c-Fos protein remains in the soma. To examine the influence of memory-modulating manipulations of the BLA on expression of these memory and plasticity-associated proteins in the rACC, male Sprague-Dawley rats were trained on an IA task and given intra-BLA infusions of either clenbuterol or lidocaine immediately after training. Findings suggest that noradrenergic stimulation of the BLA may modulate memory consolidation through effects on both synaptic proteins Arc and CaMKII alpha, but not the somatic protein c-Fos. Furthermore, protein changes observed in the rACC following BLA manipulations suggest that the influence of the BLA on synaptic proteins is not limited to those in the dorsal hippocampus.