Amperometric fructose sensor based on direct bioelectrocatalysis
Amperometric fructose sensor based on direct bioelectrocatalysis
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DOI：
10.1016/0956-5663(91)85015-o
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发表时间：
1991
影响因子：
12.6
通讯作者：
T. Ikeda;F. Matsushita;M. Senda
中科院分区：
文献类型：
--
作者：
T. Ikeda;F. Matsushita;M. Senda
通过用透析膜覆盖酶层，将来自细菌膜的果糖脱氢酶（EC 1.1.99.11）固定在碳糊电极上。果糖脱氢酶修饰的碳糊电极在不添加任何外部电子转移介体的情况下表现出对果糖的电流响应。电流响应与溶液中的氧浓度无关。在固定电极电位下测量时获得稳态电流。研究了稳态电流对电势、溶液 pH 值和温度的依赖性。在此研究的基础上，结果表明果糖脱氢酶修饰的碳糊电极可以用作无介导电流果糖传感器。使用该电极测量了水果中的d-果糖。还描述了消除l-抗坏血酸影响的方法。
Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response tod-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor.d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect ofl-ascorbic acid is also described.