Quantitative polymerase chain reaction for human herpesvirus diagnosis and measurement of Epstein-Barr virus burden in posttransplant lymphoproliferative disorder.
Quantitative polymerase chain reaction for human herpesvirus diagnosis and measurement of Epstein-Barr virus burden in posttransplant lymphoproliferative disorder.
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定量聚合酶链反应用于人疱疹病毒诊断和移植后淋巴增殖性疾病中 Epstein-Barr 病毒负荷的测量。
DOI：
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发表时间：
1997
影响因子：
9.3
通讯作者：
Richard H Scheuermann
中科院分区：
文献类型：
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作者：
Xin Bai;Gregory Hosler;Beverly Barton Rogers;D. Dawson;Richard H Scheuermann
人类疱疹病毒可引起急性疾病，如水痘或单核细胞增多症，但也可能在免疫抑制期间重新激活，导致严重或危及生命的疾病，如带状疱疹或淋巴细胞增生性疾病。我们报告了一种定量PCR方法的开发和验证，用于测量患者样本中所有八种人类疱疹病毒（HSV1, HSV2， VZV， EBV， CMV, HHV6， HHV7和KSHV）的病毒负荷。该方法使用与病毒靶标共扩增的内部标准物，允许对病毒基因组进行绝对定量（例如，病毒靶标/mL血液），并排除假阴性结果。我们证明了淋巴增生性疾病的移植患者携带的EBV病毒负担比未移植患者高3倍。没有淋巴增生性疾病的移植患者的EBV滴度介于这两个值之间。这种定量PCR方法可能有助于区分免疫抑制患者的临床显著和潜伏病毒负担。
Human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe or life-threatening illnesses such as shingles or lymphoproliferative disorders. We report the development and validation of a quantitative PCR method to measure viral burden for all eight human herpesviruses (HSV1, HSV2, VZV, EBV, CMV, HHV6, HHV7, and KSHV) in patients' samples. The method uses an internal standard that is coamplified with the viral target, allowing quantification of viral genomes in absolute terms (e.g., viral targets/mL of blood) and ruling out false-negative results. We demonstrate that transplant patients with lymphoproliferative disorder carry an EBV viral burden 3 logs higher than nontransplant patients. EBV titers in transplant patients without a lymphoproliferative disorder are between these values. This quantitative PCR method may aid in differentiating clinically significant vs latent viral burden in immunosuppressed patients.
影响因子：
20.3
作者：
Arvanitakis, L;Mesri, EA;Cesarman, E
通讯作者：
Cesarman, E
DOI：
10.1016/0167-4781(83)90056-8
发表时间：
1983-11
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
S. Kit;M. Kit;H. Qavi;D. Trkula;H. Otsuka
通讯作者：
S. Kit;M. Kit;H. Qavi;D. Trkula;H. Otsuka