Magnetic loading of tyrosinase-Fe3O4/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing
Magnetic loading of tyrosinase-Fe3O4/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing
复制标题
用于高灵敏电化学生物传感的酪氨酸酶-Fe3O4/介孔二氧化硅核/壳微球的磁负载
DOI：
10.1016/j.aca.2010.11.053
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发表时间：
2011-02-07
影响因子：
6.2
通讯作者：
Meng, Changgong
中科院分区：
文献类型：
--
作者：
Wu, Shuo;Wang, Hainan;Meng, Changgong
提出了一种利用酪氨酸酶交联介孔磁核/磁壳微球对苯酚进行磁负载和灵敏电化学检测的新方案。通过透射电子显微镜、N-2吸附/解吸等温线和磁性曲线对所制得的介孔磁性微球进行了表征，显示出较高的酶固定化能力和较强的磁性，无需额外的粘附剂即可粘附在磁性电极表面。该生物传感器对苯酚的线性响应范围为1.0 × 10(-9) ~ 1.0 × 10(-5) M，灵敏度高达78 μ a mM(-1)，检测限低至1 nM，响应速度快（小于5 s）。该方法具有简单、快速、价格低廉、电极更新方便等优点，在生物传感领域具有广阔的应用前景。(C) 2010 Elsevier B.V.版权所有
A new protocol is proposed for magnetic loading and sensitive electrochemical detection of phenol via the tyrosinase cross-linked mesoporous magnetic core/shell microspheres. The mesoporous magnetic microspheres, characterized by transmission electron microscopy, N-2 adsorption/desorption isotherms, and magnetic curve displays high capacity for enzyme immobilization and strong magnetism to adhere to the magnetic electrode surface without any additional adhesive reagent. The biosensor exhibits a wide linear response to phenol ranging from 1.0 x 10(-9) to 1.0 x 10(-5) M, a high sensitivity of 78 mu A mM(-1), a low detection limit of 1 nM, and a fast response rate (less than 5 s). The proposed method is simple, rapid, inexpensive and convenient in electrode renewal, which is recommended as a promising experimental platform for wider applications in biosensing. (C) 2010 Elsevier B.V. All rights reserved.