Role of cuticular lipids in nestmate recognition of the European hornet Vespa crabro L. (Hymenoptera, Vespidae)
Role of cuticular lipids in nestmate recognition of the European hornet Vespa crabro L. (Hymenoptera, Vespidae)
复制标题
角质层脂质在欧洲大黄蜂 Vespacrabro L.（膜翅目，胡蜂科）巢伴识别中的作用
作者：
J. Ruther;S. Sieben;B. Schricker
为了阐明欧洲胡蜂对巢友的识别，（Hypltera，Vespidae）工蜂对死亡巢友和非巢友的行为反应在生物测定中进行了测试。测试竞技场被整合在一个社会和物理上完整的巢穴中，以确保极其敏感的黄蜂的自然行为。大黄蜂对死去的非巢友的反应是攻击性行为，而死去的巢友则被容忍或忽视。溶剂萃取的非nestmate假人导致显着减少的竞争行为的反应工人。他们对提取的非巢伙伴假人的行为不再是统计上的区别对巢伙伴假人以同样的方式处理。处理提取的nestmate假人与非nestmates的表皮提取物诱导的攻击行为在响应的工人，而处理提取的nestmate假人与nestmates的表皮提取物导致显着减少的对抗行为，与未处理的非nestmates相比。对两个菌落的表皮提取物的化学组成进行统计比较，发现5种单烯、4种甲基支链烷烃、1种萜类酯和2种正烷烃的相对峰面积差异显著。杜氏腺的化学分析表明，所有16种化合物中确定的腺体也存在于表皮提取物。结果强烈表明，表皮脂质的特殊混合物在V. crabro的巢伙伴识别中起着重要作用。
Summary: In order to elucidate nestmate recognition in the European hornet Vespa crabro L. (Hymenoptera, Vespidae) the behavioral response of workers towards dead nestmates and non-nestmates was tested in a bioassay. The testing arena was integrated in a socially and physically intact nest, in an effort to ensure natural behavior of the extremely sensitive wasps. The hornets responded to dead non-nestmates with aggressive behavior, whereas dead nestmates were tolerated or ignored. Solvent extraction of non-nestmate dummies resulted in significant decrease of agonistic behavior of the responding workers. Their behavior towards extracted non-nestmate dummies was no longer statistically distinguishable from the behavior towards nestmate dummies treated in the same way. Treatment of extracted nestmate dummies with cuticular extracts of non-nestmates induced aggressive behavior in the responding workers, whereas treatment of the extracted non-nestmate dummies with cuticular extracts of nestmates lead to a significant decrease of agonistic behavior, when compared with untreated non-nestmates. ¶A statistical comparison of the two colonies with regard to the chemical composition of the cuticular extracts revealed that relative peak areas of 5 monoenes, 4 methyl branched alkanes, 1 terpenoid ester, and 2 n-alkanes differed significantly. The chemical analysis of the Dufour's gland revealed that all 16 compounds identified in the gland were also present in the cuticular extracts. ¶The results strongly indicate that particular mixtures of cuticular lipids play an important role in nestmate recognition of V. crabro.