The actin cytoskeleton is important genome replication for rotavirus internalization and RNA
The actin cytoskeleton is important genome replication for rotavirus internalization and RNA
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DOI：
10.1016/j.virusres.2019.01.003
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发表时间：
2019-04-02
期刊：
影响因子：
5
通讯作者：
Arias, Carlos F.
中科院分区：
文献类型：
--
作者：
Trejo-Cerro, Oscar;Aguilar-Hernandez, Nayeli;Arias, Carlos F.
轮状病毒的有效复制需要多种宿主因素，包括几个细胞信号通路的激活和失活。在轮状病毒感染过程中重组的细胞结构之一是肌动蛋白细胞骨架。在这项工作中，我们报告了肌动蛋白微丝的动力学在病毒生命周期的不同阶段，特别是在感染后6h的病毒内化和病毒RNA合成期间是重要的。我们的结果表明，肌动蛋白结合蛋白α-肌动蛋白4和DIAPH，以及Rho家族的小GTP酶CDc42是有效进入病毒所必需的，而GTP酶rac1是最大限度合成病毒RNA所必需的。
Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin microfilaments are important at different stages of the virus life cycle, specifically, during virus internalization and viral RNA synthesis at 6 h post-infection. Our results show that the actin-binding proteins alpha-actinin 4 and Diaph, as well as the Rho-family small GTPase Cdc42 are necessary for an efficient virus entry, while GTPase Rac1 is required for maximal viral RNA synthesis.