The environmental enrichment effect upon cortical growth is neutralized by concomitant pharmacological suppression of active sleep in female rats
The environmental enrichment effect upon cortical growth is neutralized by concomitant pharmacological suppression of active sleep in female rats
复制标题
环境富集对皮质生长的影响被雌性大鼠主动睡眠的同时药理抑制所抵消
DOI：
10.1016/0006-8993(83)90639-x
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
H. Uylings
中科院分区：
文献类型：
--
作者：
M. Mirmiran;H. Uylings
为了验证在富集条件下饲养的大鼠中观察到的主动（REM）睡眠有助于增强大脑发育的假设，3组雌性大鼠在出生后28天被分配到以下组中：（a）每天2.5小时暴露于富集环境，然后立即剥夺主动睡眠（可乐定注射）； (b) 每日环境富集加上仅车辆；或（c）每日注射媒介物，但在标准实验室条件下连续饲养。此外，为了控制对皮质生长可能的直接药理作用，在标准条件下对另一组大鼠长期注射可乐定或盐水。 65 天时处死所有动物，并将大脑解剖成不同区域进行称重。与标准盐水对照组相比，浓缩盐水组的大脑总重量显着增加（主要是由于大脑皮层较重）。相比之下，富含可乐定的大鼠与标准盐水组没有表现出差异。此外，在标准环境中饲养的注射可乐定和注射生理盐水的大鼠之间没有发现显着差异。结果表明，主动睡眠和中枢 NA 活动在环境刺激对特定大脑区域的生长促进作用中发挥着重要作用。
In order to examine the hypothesis that active (REM) sleep contributes to the enhanced brain development which is observed in rats reared under enriched conditions, 3 groups of female rats were assigned at 28 days after birth to either: (a) daily 2.5 h exposure to an enriched environment, followed immediately by active sleep deprivation (clonidine injection); (b) daily environmental enrichment plus vehicle only; or (c) daily vehicle injection, but continuous rearing under standard laboratory conditions. In addition, so as to control for a possible direct pharmacological effect upon cortical growth, another series of rats was chronically injected with clonidine or saline under standard conditions. At 65 days of age all the animals were sacrificed and the brains dissected into different regions for weighing. A significant increase in total brain weight (mainly due to a heavier cerebral cortex) was found in the enriched-saline group as compared with the standard-saline controls. In contrast, the enriched-clonidine rats showed no differences from the standard-saline group. Moreover, no significant differences were found between clonidine- and saline-injected rats reared in the standard environment. The results indicate that active sleep and central NA activity play a role in growth-promoting effects of environmental stimulation upon specific brain regions.